Laboratory Medicine, Liangshan People's Hospital, Liangshan, China.
Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):6944-6950. doi: 10.26355/eurrev_201908_18734.
The aim of this study was to explore the expression of long non-coding RNA (lncRNA) KCNQ1OT1 in non-small cell lung cancer (NSCLC), and to elucidate its clinical significance.
The quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA KCNQ1OT1 in NSCLC tissues and para-cancer tissues (5 cm or above away from the tumor). The relation between lncRNA KCNQ1OT1 expression and the clinical-pathological data was analyzed by the multivariate logistic regression analysis. Furthermore, the survival analysis was performed by the Kaplan-Meier method.
The expression of lncRNA KCNQ1OT1 increased significantly in NSCLC tissues than that of in para-cancer tissues. According to the median expression of lncRNA KCNQ1OT1, NSCLC patients were divided into two groups, including high expression group and low expression group. Meanwhile, the lncRNA KCNQ1OT1 expression was correlated with tumor size, tumor node metastasis (TNM) staging, and lymph node metastasis of NSCLC patients. Both univariate analysis and multivariate analysis indicated that the high expression of lncRNA KCNQ1OT1 was closely related to TNM staging and lymph node metastasis. In addition, the Kaplan-Meier analysis showed that the overall survival and progression-free survival time of patients with higher lncRNA KCNQ1OT1 expression were significantly worse than those with lower lncRNA KCNQ1OT1 expression.
LncRNA KCNQ1OT1 might contribute to the development of NSCLC.
本研究旨在探讨长链非编码 RNA（lncRNA）KCNQ1OT1 在非小细胞肺癌（NSCLC）中的表达，并阐明其临床意义。
采用实时定量聚合酶链反应（qRT-PCR）检测 NSCLC 组织及癌旁组织（距肿瘤 5cm 以上）中 lncRNA KCNQ1OT1 的表达。采用多因素 logistic 回归分析 lncRNA KCNQ1OT1 表达与临床病理资料的关系。进一步采用 Kaplan-Meier 法进行生存分析。
lncRNA KCNQ1OT1 在 NSCLC 组织中的表达明显高于癌旁组织。根据 lncRNA KCNQ1OT1 的中位表达，将 NSCLC 患者分为高表达组和低表达组。同时，lncRNA KCNQ1OT1 的表达与 NSCLC 患者的肿瘤大小、肿瘤淋巴结转移（TNM）分期和淋巴结转移相关。单因素和多因素分析均表明，lncRNA KCNQ1OT1 的高表达与 TNM 分期和淋巴结转移密切相关。此外，Kaplan-Meier 分析显示，lncRNA KCNQ1OT1 高表达患者的总生存时间和无进展生存时间明显短于 lncRNA KCNQ1OT1 低表达患者。
lncRNA KCNQ1OT1 可能参与 NSCLC 的发生发展。
