Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao, China.
Department of Endocrinology and Metabolism, Affiliated Hospital of Qingdao University, Qingdao, China.
Thorac Cancer. 2018 May;9(5):523-531. doi: 10.1111/1759-7714.12599. Epub 2018 Mar 5.
Lung cancer (LC) is the most common malignancy in the world. Many long non-coding RNAs (lncRNAs) have been reported to be associated with LC; however, the function of KCNQ1OT1 in LC requires exploration.
We conducted in silico analysis with data from The Cancer Genome Atlas to investigate the association between KCNQ1OT1 and LC. A Kaplan-Meier plotter was used to analyze the function of KCNQ1OT1 on LC patient prognosis. Quantitative reverse transcription-PCR (qRT-PCR) was performed to confirm previous results. An A549 lung cancer cell was transfected with pcDNA-KCNQ1OT1, and methyl thiazolyl tetrazolium assay was performed to investigate the function of KCNQ1OT1 on cell proliferation. in vivo assay was performed with nude mice.
Bioinformatics analysis and qRT-PCR indicated that KCNQ1OT1 expression was higher in stage I LC patients (P < 0.01), and survival analysis showed that high expression of KCNQ1OT1 in LC patients was associated with better prognosis (P < 0.05). qRT-PCR showed a negative correlation between KCNQ1OT1 and Ki67 expression and tumor size (P < 0.01), which indicated that KCNQ1OT1 is associated with tumor growth in LC. There was no significant correlation between KCNQ1OT1 level and lymph node metastasis (P > 0.05). KCNQ1OT1 overexpression significantly inhibited cell proliferation and tumor growth in vitro and in vivo (P < 0.05).
Our preliminary data showed that KCNQ1OT1 is overexpressed in early stage LC and is correlated with better prognosis in LC patients, possibly by suppressing cell proliferation.
肺癌（LC）是世界上最常见的恶性肿瘤。许多长链非编码 RNA（lncRNA）已被报道与 LC 相关；然而，KCNQ1OT1 在 LC 中的功能仍需探索。
我们使用来自癌症基因组图谱的数据进行了计算机分析，以研究 KCNQ1OT1 与 LC 之间的关联。使用 Kaplan-Meier 绘图仪分析 KCNQ1OT1 对 LC 患者预后的功能。进行了定量逆转录-PCR（qRT-PCR）以验证先前的结果。用 pcDNA-KCNQ1OT1 转染 A549 肺癌细胞，并进行甲基噻唑基四唑测定以研究 KCNQ1OT1 对细胞增殖的功能。使用裸鼠进行体内试验。
生物信息学分析和 qRT-PCR 表明，I 期 LC 患者的 KCNQ1OT1 表达更高（P<0.01），生存分析表明，LC 患者中 KCNQ1OT1 的高表达与更好的预后相关（P<0.05）。qRT-PCR 显示 KCNQ1OT1 与 Ki67 表达和肿瘤大小呈负相关（P<0.01），这表明 KCNQ1OT1 与 LC 中的肿瘤生长有关。KCNQ1OT1 水平与淋巴结转移之间无显著相关性（P>0.05）。KCNQ1OT1 过表达可显著抑制体外和体内细胞增殖和肿瘤生长（P<0.05）。
我们的初步数据表明，KCNQ1OT1 在早期 LC 中过度表达，并与 LC 患者的更好预后相关，可能通过抑制细胞增殖。
