IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy.
Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.
Molecules. 2019 Sep 4;24(18):3217. doi: 10.3390/molecules24183217.
Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate] is an isothiocyanate extracted from seeds. It is an antioxidant known for several biological properties useful in the treatment of neurodegenerative diseases. Several neurodegenerative disorders such as Parkinson's and Alzheimer's diseases are linked to dysfunctional mitochondria due to the resulting increase of Reactive Oxygen Species (ROS). Stem cell-based therapeutic treatments in neurodegenerative diseases provide an alternative strategy aimed to replace the impaired tissue. In this study were investigated the deregulated genes involved in mitophagy in the human periodontal ligament stem cells pretreated with moringin. The RNA-seq study reveals the downregulation of with a fold change (FC) of -0.56, such as the genes involved in the phagophore formation ( FC: -0.73, FC: -0.52, FC: -0.70, FC: -0.39). The moringin pretreatment downregulates the pro-apoptotic gene (-0.66) and upregulates the anti-apoptotic genes (FC: 1.35) and (FC: 0.36). The downregulation of the most of the caspases ( FC: -1.43, FC: -0.18, FC: -1.34, FC: -0.46, FC: -0.65) implies the inactivation of the apoptotic process. Our results suggest that mitochondrial dysfunctions induced by oxidative stress can be inhibited by moringin pretreatment in human periodontal ligament stem cells (hPDLSCs).
柚皮苷[4-（α-L-鼠李糖氧基）苄基异硫氰酸酯]是从种子中提取的异硫氰酸酯。它是一种抗氧化剂，具有几种生物特性，可用于治疗神经退行性疾病。几种神经退行性疾病，如帕金森病和阿尔茨海默病，由于活性氧物种（ROS）的增加，与功能失调的线粒体有关。基于干细胞的神经退行性疾病治疗提供了一种替代策略，旨在替代受损组织。在这项研究中，研究了柚皮苷预处理的人牙周膜干细胞中线粒体自噬相关的失调基因。RNA-seq 研究显示，与吞噬体形成相关的基因下调（FC：-0.73，FC：-0.52，FC：-0.52，FC：-0.70，FC：-0.39）。柚皮苷预处理下调促凋亡基因（-0.66）并上调抗凋亡基因（FC：1.35）和（FC：0.36）。大多数半胱天冬酶的下调（FC：-1.43，FC：-0.18，FC：-1.34，FC：-0.46，FC：-0.65）暗示凋亡过程失活。我们的结果表明，氧化应激诱导的线粒体功能障碍可以通过柚皮苷预处理抑制人牙周膜干细胞（hPDLSCs）中的线粒体功能障碍。
