Wen Yanlong, Li Wenyun, Su Rongzhen, Yang Min, Zhang Nan, Li Ximing, Li Lingfei, Sheng Jun, Tian Yang
College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.
National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, China.
Front Microbiol. 2022 Jun 8;13:925291. doi: 10.3389/fmicb.2022.925291. eCollection 2022.
Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate] is an isothiocyanate from seeds. It is the bioactivated form of the glucosinolate precursor glucomoringin with various health benefits. However, few studies have examined the antibacterial activity of moringin. This study aimed to investigate the antimicrobial activity and mechanism of moringin against . The minimum inhibitory concentration (MIC), and growth curves were used to evaluate the bacteriostatic effect of moringin against . Transcriptome analysis by RNA sequencing was performed to elucidate the underlying mechanism of moringin against . The transcriptome results were validated. The results showed that moringin inhibited the growth of with a MIC of 400 μM. RNA sequencing results showed that the differences in the expression of genes related to the cell wall and membrane biosynthesis, phosphotransferase system (PTS), oxidative stress, energy metabolism, and DNA binding were significantly affected. As with the transcriptome results, the results of the mechanism verification found that moringin damaged the integrity of the cell wall and cell membrane, stimulated oxidative stress, interfered with energy metabolism and DNA replication, and finally led to the death of . The present study provides evidence that moringin exhibits strong antimicrobial activity against and insight into its potential mechanism.
辣木素[4-(α-L-鼠李糖氧基)苄基异硫氰酸酯]是一种从种子中提取的异硫氰酸酯。它是硫代葡萄糖苷前体葡萄糖异硫氰酸酯的生物活化形式，具有多种健康益处。然而，很少有研究考察辣木素的抗菌活性。本研究旨在探究辣木素对[具体细菌名称未给出]的抗菌活性及其作用机制。采用最低抑菌浓度（MIC）和生长曲线来评估辣木素对[具体细菌名称未给出]的抑菌效果。通过RNA测序进行转录组分析，以阐明辣木素对[具体细菌名称未给出]作用的潜在机制，并对转录组结果进行了验证。结果表明，辣木素对[具体细菌名称未给出]具有抑制生长作用，MIC为400μM。RNA测序结果显示，与细胞壁和细胞膜生物合成、磷酸转移酶系统（PTS）、氧化应激、能量代谢及DNA结合相关的基因表达差异受到显著影响。与转录组结果一致，作用机制验证结果发现，辣木素破坏了细胞壁和细胞膜的完整性，刺激了氧化应激，干扰了能量代谢和DNA复制，最终导致[具体细菌名称未给出]死亡。本研究提供了证据表明辣木素对[具体细菌名称未给出]具有强大的抗菌活性，并深入了解了其潜在机制。
