IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, Contrada Casazza, 98124, Messina, Italy.
Department of medical, oral and biotechnological sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
Sci Rep. 2018 Jun 14;8(1):9153. doi: 10.1038/s41598-018-27492-0.
The therapeutic strategies for neurodegenerative diseases still represent a vast research field because of the lack of targeted, effective and resolutive treatment for neurodegenerative diseases. The use of stem cell-based therapy is an alternative approach that could lead to the replacement of damaged neuronal tissue. For this purpose, adult mesenchymal stem cells (MSC), including periodontal ligament stem cells (PDLSCs), could be very useful for their differentiation capacity, easy isolation and the ability to perform an autologous implant. The aim of this work was to test whether the Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate; GMG-ITC], an isothiocyanate extracted from Moringa oleifera seeds, was able to induce PDLSCs toward neural progenitor differentiation. Next-generation transcriptomics sequencing showed that moringin treatment increased the expression of genes involved in neuron cortical development and in particular in neuron belonging to upper and deep cortical layers. Moreover, moringin treatment upregulated genes involved in osteogenesis and adipogenesis although with a lower fold change compared to upregulated genes involved in neuronal differentiation. Finally, moringin did not induce the expression of oncogenes resulting in a safe treatment.
由于缺乏针对神经退行性疾病的靶向、有效和决定性的治疗方法，神经退行性疾病的治疗策略仍然是一个广阔的研究领域。基于干细胞的治疗方法是一种替代方法，可以导致受损神经元组织的替代。为此，包括牙周膜干细胞（PDLSCs）在内的成体间充质干细胞（MSC），由于其分化能力、易于分离以及进行自体植入的能力，可能非常有用。这项工作的目的是测试从辣木叶种子中提取的异硫氰酸酯——Moringin [4-（α-L-鼠李糖氧基）苄基异硫氰酸酯；GMG-ITC]，是否能够诱导 PDLSCs 向神经祖细胞分化。下一代转录组测序显示，Moringin 处理增加了参与皮质神经元发育的基因的表达，特别是涉及皮质上层和深层神经元的基因。此外，Moringin 处理上调了参与成骨和成脂的基因，尽管与参与神经元分化的基因上调倍数相比，这些基因的上调倍数较低。最后，Moringin 没有诱导致癌基因的表达，从而实现了安全的治疗。
