Bassett D J, Bowen-Kelly E, Brewster E L, Elbon C L, Reichenbaugh S S, Bunton T, Kerr J S
Department of Environmental Health Sciences, Johns Hopkins University Medical Institutions, Baltimore, Maryland.
Lung. 1988;166(6):355-69. doi: 10.1007/BF02714068.
In this study inflammatory responses were determined in rat lungs 0, 1, 3, and 8 days following single 2- and 4-hr exposures to 1.8 ppm ozone. Analysis of lavage fluid immediately following exposure demonstrated enhanced lactate dehydrogenase activity and decreased numbers of lavageable macrophages but no alterations in albumin content. Similar analyses at one day postexposure demonstrated 282% and 456% increases in albumin content and enhanced numbers of lavageable neutrophils from a control value of 0.01 +/- 0.01 to 0.27 +/- 0.10 and 0.78 +/- 0.11 million cells per lung for 2-hr and 4-hr exposures, respectively. The observed increased levels of albumin were also present at 3 days, at which time the number of lavageable neutrophils was not significantly different than control. At both one and 3 days postexposure, lavageable lymphocytes were significantly increased 10-fold from a control value of 0.03 +/- 0.01 million cells per lung. However, the number of lavageable macrophages was unaltered on day 1, but enhanced on day 3, giving values of 0.67 +/- 0.05 (control), 2.25 +/- 0.46 (2 hr), and 2.70 +/- 1.05 (4 hr) million cells per lung. By 8 days both inflammatory cell numbers and albumin levels had returned to control values. Since these data demonstrated different time courses for each inflammatory cell type, this reversible model of acute lung injury should be useful for establishing possible involvement of these cells in processes of lung injury.
在本研究中，在大鼠单次暴露于1.8 ppm臭氧2小时和4小时后的0、1、3和8天，测定其肺部的炎症反应。暴露后立即对灌洗液进行分析，结果显示乳酸脱氢酶活性增强，可灌洗巨噬细胞数量减少，但白蛋白含量无变化。暴露后一天进行的类似分析表明，白蛋白含量分别增加了282%和456%，可灌洗中性粒细胞数量增加，对于2小时和4小时的暴露，每只肺中可灌洗中性粒细胞数量从对照值0.01±0.01分别增加到0.27±0.10和0.78±0.11百万个细胞。在3天时也观察到白蛋白水平升高，此时可灌洗中性粒细胞数量与对照无显著差异。在暴露后1天和3天，可灌洗淋巴细胞数量均显著增加，从对照值每只肺0.03±0.01百万个细胞增加了10倍。然而，可灌洗巨噬细胞数量在第1天未改变，但在第3天增加，每只肺的细胞数量分别为0.67±0.05（对照）、2.25±0.46（2小时）和2.70±1.05（4小时）百万个细胞。到8天时，炎症细胞数量和白蛋白水平均恢复到对照值。由于这些数据显示每种炎症细胞类型具有不同的时间进程，这种急性肺损伤的可逆模型应有助于确定这些细胞在肺损伤过程中可能的参与情况。
