Selçuk Muhammet Lütfi, Tıpırdamaz Saadettin
Department of Physiotherapy and Rehabilitation, Faculty of Health Sciences, Karamanoglu Mehmetbey University, Karaman, Turkey.
Department of Anatomy, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.
Anat Histol Embryol. 2020 Jan;49(1):90-96. doi: 10.1111/ahe.12489. Epub 2019 Sep 6.
The aim of this research was to determine brain, cerebral hemispheres and cerebellum volume and volume ratios by using stereological methods and investigate morphological differences between female and male New Zealand rabbits. The study was applied on 14-month old (10 male and 10 female) New Zealand rabbits. The materials removed from the cavum cranii using dorsal approach. After following routine histological procedure, paraffin blocks were prepared and cut every seventieth section at 10 μm thickness. Slides were stained with Crossmon's triple stain and photographed. The sectional images obtained were transferred to ImageJ program to estimate grey and white matter volume on cerebral hemispheres and cerebellum with principle of Cavalieri. According to results, there was no asymmetry on the left and right cerebral hemispheres of New Zealand rabbits. In the total hemisphere volume calculated by Cavalieri principle, grey and white matter ratio was 81.57% and 18.43% in female, 82.80% and 17.20% in male. It was found that the white matter was significantly higher in females than males in cerebral hemispheres (p < .05). Also, it was found that grey and white matter ratio in total cerebellum volume was 67.82% and 32.18% in female, 67.94% and 32.06% in male respectively. It was determined that the females' white matter was larger than male rabbits in cerebellum (p < .05). It is thought that morphometric data obtained from this study will contribute to the existing anatomical knowledge and also considered as reference values in the clinical sciences.
本研究的目的是采用体视学方法测定新西兰兔的脑、大脑半球和小脑体积及体积比，并研究雌雄新西兰兔之间的形态学差异。该研究应用于14月龄的新西兰兔（10只雄性和10只雌性）。采用背侧入路从颅腔取出材料。按照常规组织学程序处理后，制备石蜡块，并以10μm厚度每隔七十张切片。切片用克罗斯蒙三色染色并拍照。将获得的切片图像转移到ImageJ程序中，根据卡瓦列里原理估算大脑半球和小脑的灰质和白质体积。结果显示，新西兰兔左右大脑半球不存在不对称性。根据卡瓦列里原理计算的全脑半球体积中，雌性的灰质和白质比例分别为81.57%和18.43%，雄性为82.80%和17.20%。结果发现，雌性大脑半球的白质显著高于雄性（p < 0.05）。此外，还发现雌性小脑总体积中的灰质和白质比例分别为67.82%和32.18%，雄性为67.94%和32.06%。结果确定，雌性小脑的白质大于雄性兔（p < 0.05）。认为本研究获得的形态测量数据将有助于现有的解剖学知识，也可作为临床科学中的参考值。
