Çolakoğlu Fatma, Selçuk Muhammet Lütfi
Department of Nutrition and Dietetics, Faculty of Health Sciences, Karamanoğlu Mehmetbey University, 70200 Karaman, Turkey.
Department of Physiotherapy and Rehabilitation, Faculty of Health Sciences, Karamanoğlu Mehmetbey University, 70200 Karaman, Turkey.
J Vet Res. 2020 Oct 15;64(4):597-607. doi: 10.2478/jvetres-2020-0064. eCollection 2020 Dec.
Sunset Yellow FCF (SY), used frequently in ready-made foods, cosmetics, and the pharmaceutical industry, may cause many health problems. This study is intended to evaluate the morphological and cellular effects of SY on the embryonic chicken immune system throughout incubation.
Babcock white leghorn eggs were randomly divided into four groups. Besides a control group, there were three treatment groups which received a single injection of 200, 1,000, or 2,000 ng of SY into the air sac just before incubation. The eggs were opened on the 10, 13, 16, and 21 days of incubation. Samples of the bursa of Fabricius, thymus, and spleen were taken from embryos. Serial sections of 5 μm thickness were stained with histological methods and routine histological procedures were performed.
An increase in the spleen volume was determined as the hatching time of the chicks approached. The highest eosinophil ratio was found in the SY and SY groups (P < 0.05), where the most significant change was developmental retardation in the thymus. In the bursa of Fabricius, there was less lymphocyte accumulation and eosinophilic cell infiltration with increasing doses.
It was concluded that administered SY has undesired effects on embryonic development of the bursa of Fabricius, spleen, and thymus, and on spleen volume.
日落黄FCF（SY）常用于即食食品、化妆品和制药行业，可能会引发多种健康问题。本研究旨在评估SY在整个孵化过程中对鸡胚胎免疫系统的形态学和细胞效应。
将巴布考克白来航鸡蛋随机分为四组。除对照组外，还有三个处理组，在孵化前向气囊中单次注射200、1000或2000纳克的SY。在孵化的第10、13、16和21天打开鸡蛋。从胚胎中采集法氏囊、胸腺和脾脏样本。制作5微米厚的连续切片，采用组织学方法染色并进行常规组织学程序。
随着雏鸡孵化时间临近，脾脏体积增大。在SY和SY组中发现嗜酸性粒细胞比例最高（P<0.05），其中最显著的变化是胸腺发育迟缓。在法氏囊中，随着剂量增加，淋巴细胞聚集减少，嗜酸性细胞浸润减少。
得出的结论是，施用SY对法氏囊、脾脏和胸腺的胚胎发育以及脾脏体积有不良影响。
