School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China.
The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou 213164, Jiangsu, P. R. China.
J Nanosci Nanotechnol. 2020 Apr 1;20(4):2087-2094. doi: 10.1166/jnn.2020.17189.
The wound of diabetic foot is difficult to heal, and in severe cases, diabetic patients have to undergo limb amputation even after wound management. Hyperglycemia-caused vascular dysfunction and serious wound bacterial infection are the two main causes of unhealed diabetic foot ulcers. Therefore, it would be of great benefit to rescue stalled healing in diabetic wound if the blood glucose concentration can be efficiently decreased while controlling the bacterial infection in diabetic wound. Herein, we report glucose oxidase (GOx)-loaded antimicrobial peptide hydrogels and investigate their potential as diabetic wound dressings. The antimicrobial hydrogels are formed by the selfassembly of a heptapeptide (IKYLSVN) driven by intermolecular hydrogen bonding and - stacking interaction. The loaded GOx can convert glucose into hydrogen peroxide (H₂O₂), thus reducing blood glucose concentration of diabetic patients. At the same time, the produced H₂O₂ is antimicrobial and can combat bacterial infection in diabetic wound together with the antimicrobial hydrogels. Microscopic morphology observation of hydrogel shows uniform fibers with approximately 40 nm in size. The embedding rate can still reach 90% when the loading rate of the hydrogel loaded glucose oxidase is 12%. GOx is released by the swelling of the hydrogel based on the mechanism of free diffusion, and the cumulative release rate reaches 55%. Moreover, experiments showed that our GOx-loaded peptide hydrogel exhibits outstanding antimicrobial activity while can efficiently reduce glucose concentration of blood samples, showing great potential as promising diabetic wound dressings.
糖尿病足的伤口难以愈合，在严重的情况下，即使进行了伤口管理，糖尿病患者也不得不进行截肢。高血糖引起的血管功能障碍和严重的伤口细菌感染是导致糖尿病足溃疡难以愈合的两个主要原因。因此，如果能在控制糖尿病伤口细菌感染的同时，有效降低血糖浓度，将有助于挽救糖尿病伤口的愈合停滞。在这里，我们报告了载葡萄糖氧化酶（GOx）的抗菌肽水凝胶，并研究了它们作为糖尿病伤口敷料的潜力。抗菌水凝胶是由七肽（IKYLSVN）通过分子间氢键和π-π堆积相互作用自组装形成的。负载的 GOx 可以将葡萄糖转化为过氧化氢（H₂O₂），从而降低糖尿病患者的血糖浓度。同时，产生的 H₂O₂具有抗菌作用，可与抗菌水凝胶一起对抗糖尿病伤口中的细菌感染。水凝胶的微观形貌观察显示出约 40nm 大小的均匀纤维。当水凝胶负载葡萄糖氧化酶的负载率为 12%时，其包埋率仍可达到 90%。GOx 基于自由扩散的机制通过水凝胶的溶胀释放，累积释放率达到 55%。此外，实验表明，我们的载 GOx 肽水凝胶表现出优异的抗菌活性，同时可以有效地降低血液样本中的葡萄糖浓度，显示出作为有前途的糖尿病伤口敷料的巨大潜力。
