Faculty of Materials Science and Chemical Engineering, Ningbo University , Ningbo 315211, China.
Division of Surgery and Interventional Science, University College London , London HA7 4LP, U.K.
ACS Appl Mater Interfaces. 2017 Nov 1;9(43):37563-37574. doi: 10.1021/acsami.7b09395. Epub 2017 Oct 23.
pH and glucose dual-responsive injectable hydrogels were prepared through the cross-linking of Schiff's base and phenylboronate ester using phenylboronic-modified chitosan, poly(vinyl alcohol) and benzaldehyde-capped poly(ethylene glycol). Protein drugs and live cells could be incorporated into the hydrogels during the in situ cross-linking, displaying sustained and pH/glucose-triggered drug release from the hydrogels and cell viability and proliferation in the three-dimensional hydrogel matrix as well. Hence, the hydrogels with insulin and fibroblasts were considered as bioactive dressings for diabetic wound healing. A streptozotocin-induced diabetic rat model was used to evaluate the efficacy of hydrogel dressings in wound repair. The results revealed that the incorporation of insulin and L929 in the hydrogels could promote neovascularization and collagen deposition and enhance the wound-healing process of diabetic wounds. Thus, the drug- and cell-loaded hydrogels have promising potential in wound healing as a medicated system for various therapeutic proteins and live cells.
pH 和葡萄糖双重响应型可注射水凝胶是通过席夫碱和苯硼酸酯的交联制备的，使用苯硼酸修饰的壳聚糖、聚乙烯醇和苯甲醛封端的聚乙二醇。在原位交联过程中，可以将蛋白质药物和活细胞掺入水凝胶中，水凝胶具有从水凝胶中持续和 pH/葡萄糖触发的药物释放以及三维水凝胶基质中的细胞活力和增殖的特性。因此，含有胰岛素和成纤维细胞的水凝胶被认为是用于糖尿病伤口愈合的生物活性敷料。使用链脲佐菌素诱导的糖尿病大鼠模型评估水凝胶敷料在伤口修复中的功效。结果表明，胰岛素和 L929 的掺入可以促进新血管生成和胶原沉积，并增强糖尿病伤口的愈合过程。因此，载药和载细胞水凝胶在伤口愈合方面具有很大的应用潜力，可作为各种治疗性蛋白质和活细胞的药物输送系统。
