Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, South Korea.
Nanoscale. 2019 Nov 21;11(45):21724-21727. doi: 10.1039/c9nr05159g.
Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.
尽管参与生命过程的各种内源性生物分子是细胞生物学的主要研究对象，但仍缺乏在不标记的情况下研究活细胞内生物分子的合适方法。在此，我们描述了一种基于近红外（NIR）表面增强拉曼散射（SERS）的粒子跟踪技术，该技术可在活细胞内收集化学信息，以监测其细胞内动力学。该宽场 SERS 成像光谱系统在高空间分辨率（512×512 像素）下实现了高时间分辨率（200 ms），可用于单个活细胞。通过高时空分辨率和信号背景比，我们表明活细胞内细胞内货物的拉曼信号是间歇性观察到的，并定位于囊泡水平。
