乳液液滴结晶度可降低健康男性餐后血浆三酰甘油反应：一项随机双盲交叉急性餐食研究。

Emulsion Droplet Crystallinity Attenuates Postprandial Plasma Triacylglycerol Responses in Healthy Men: A Randomized Double-Blind Crossover Acute Meal Study.

机构信息

Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.

出版信息

J Nutr. 2020 Jan 1;150(1):64-72. doi: 10.1093/jn/nxz207.

DOI:10.1093/jn/nxz207

PMID:31495898

Abstract

BACKGROUND

The presence of triacylglycerol (TAG) cystallinity is assumed to influence digestibility and postprandial lipemia (PPL), although studies to date are limited.

OBJECTIVE

This study aimed to investigate whether the presence of solid fat compared with undercooled liquid oil, specifically, plays a role in determining PPL by comparing emulsion droplets differing only in terms of physical state.

METHODS

Ten percent palm stearin and 0.4% sorbitan monostearate emulsions were tempered to contain identically sized, charged, and shaped (spherical) undercooled liquid (LE) compared with partially crystalline solid (SE; mean ± SEM: 33.2% ± 0.03% solid fat at 37°C) droplets. Fifteen healthy fasting adult men (mean ± SD age: 27.5 ± 5.7 y; BMI: 24.1 ± 2.5 kg/m2) consumed 500 mL of each emulsion on separate occasions and plasma TAG concentrations, particle size of the plasma chylomicron-rich fraction (CMRF), and fatty acid (FA) composition of the CMRF-TAG were serially determined in a 6-h postprandial randomized double-blind crossover acute meal study. Changes from baseline values were analyzed by repeated-measures ANOVA.

RESULTS

An earlier (2 compared with 3 h, P < 0.05) significant rise, a 39.9% higher mean postprandial TAG change from baseline (P = 0.08), and higher peak concentration (mean ± SEM: 1.47 ± 0.19 compared with 1.20 ± 0.15 mmol/L, P = 0.04) and iAUC (1.95 ± 0.39 compared with 1.45 ± 0.31 mmol/L × h, P = 0.03) values were observed for LE compared with SE. The compositions of the CMRF-TAG FAs shifted toward those of the ingested palm stearin by 4 h but did not differ between SE and LE (P = 0.90). Nor were there differences in postprandial changes in CMRF particle size (P = 0.79) or nonesterified FAs (P = 0.72) based on lipid physical state.

CONCLUSIONS

Despite their identical compositions and colloidal properties, differences in lipid absorption were observed between SE and LE in healthy adult men. This is direct evidence that TAG physical state contributes to PPL, with the presence of solid fat having an attenuating influence.This trial was registered at clinicaltrials.gov as NCT03515590.

摘要

背景

三酰基甘油（TAG）结晶的存在被认为会影响消化率和餐后血脂（PPL），尽管迄今为止的研究有限。

目的

本研究旨在通过比较仅在物理状态上有所不同的乳液液滴，研究与过冷液体油相比，固体脂肪的存在是否对确定 PPL 起作用。

方法

将 10%棕榈硬脂和 0.4%山梨糖醇单硬脂酸酯乳液调温至含有相同大小、相同电荷和相同形状（球形）的过冷液体（LE），与部分结晶固体（SE；在 37°C 下的平均 ± SEM：33.2%±0.03%固体脂肪）液滴。15 名健康空腹成年男性（平均 ± 标准差年龄：27.5 ± 5.7 岁；BMI：24.1 ± 2.5 kg/m2）分别在不同时间内饮用 500 mL 每种乳液，在 6 小时的餐后随机双盲交叉急性餐研究中连续测定血浆 TAG 浓度、富含乳糜微粒的血浆颗粒大小（CMRF）和 CMRF-TAG 的脂肪酸（FA）组成。通过重复测量方差分析分析从基线值的变化。

结果

与 3 小时相比，LE 在 2 小时（P<0.05）时出现更早的显著升高，餐后 TAG 变化的平均变化比基线高 39.9%（P=0.08），峰值浓度更高（平均值 ± SEM：1.47±0.19 与 1.20±0.15 mmol/L，P=0.04）和 iAUC（1.95±0.39 与 1.45±0.31 mmol/L×h，P=0.03）。与 SE 相比，LE 观察到 CMRF-TAG FAs 的组成向摄入的棕榈硬脂转移，但在 SE 和 LE 之间没有差异（P=0.90）。基于脂质物理状态，CMRF 颗粒大小（P=0.79）或非酯化 FAs（P=0.72）的餐后变化也没有差异。