Saleh Ahmed, Anwar Mamdouh M, Zayed Ahmed E, Ezz Eldeen Manal El Sayed, Afifi Gamal, Alnashiri Hassien M, Gomaa Asmaa Ms, Abd-Elkareem Mahmoud, Abou-Elhamd Alaa Sayed, Shaheen Emad S, Mohamed Ghada A, Hetta Helal F, Kotb Ahmed M
Department of Physics, Faculty of Science, Jazan University, Jazan, KSA.
Exploratory Center of Science and Technology , Cairo, Egypt.
Diabetes Metab Syndr Obes. 2019 Aug 7;12:1339-1347. doi: 10.2147/DMSO.S209856. eCollection 2019.
Type 2 diabetes (T2D) is a widely distributed disease that affects large population worldwide. This study aimed to verify the role of (GB) extract and magnetized water (MW) on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rats.
T2D was induced by feeding the rats on a high-fat diet (20% fat, 45% carbohydrate, 22% protein) for eight weeks followed by intra-peritoneal injection of a single low dose of streptozotocin (25mg/Kg). Forty rats were randomly assigned to four groups (n=10 rats) as follows: non treated control and three diabetic groups. One diabetic group served as a positive control (diabetic), while the other two groups were orally administered with water extract of GB leaves (0.11 g/kg/day) and MW (600 gauss) for four weeks, respectively.
The β-cell mass and insulin expression in these cells increased markedly after both treatments, particularly in GB treated group. In addition, the immune-expression of the two antioxidant enzymes; glutathione and superoxide dismutase 2 (SOD2) in the pancreatic tissue demonstrated a down-regulation in GB and MW treated groups as compared with the diabetic group.
A four-week treatment of GB and MW protected pancreatic β-cell cells and improved their insulin expression and antioxidant status in type 2 diabetic rats.
2型糖尿病（T2D）是一种广泛分布的疾病，影响着全球大量人口。本研究旨在验证（GB）提取物和磁化水（MW）对2型糖尿病大鼠胰腺β细胞存活率和功能的作用。
通过给大鼠喂食高脂饮食（20%脂肪、45%碳水化合物、22%蛋白质）八周，随后腹腔注射单次低剂量链脲佐菌素（25mg/Kg）诱导T2D。40只大鼠随机分为四组（每组n = 10只大鼠），如下：未治疗的对照组和三个糖尿病组。一个糖尿病组作为阳性对照（糖尿病组），而另外两个组分别口服GB叶水提取物（0.11g/kg/天）和MW（600高斯）四周。
两种治疗后，这些细胞中的β细胞量和胰岛素表达均显著增加，尤其是在GB治疗组。此外，与糖尿病组相比，胰腺组织中两种抗氧化酶谷胱甘肽和超氧化物歧化酶2（SOD2）的免疫表达在GB和MW治疗组中呈下调。
对2型糖尿病大鼠进行四周的GB和MW治疗可保护胰腺β细胞，并改善其胰岛素表达和抗氧化状态。
