Ayan Durmuş, Şeneş Mehmet, Çaycı Ayşe Banu, Söylemez Sibel, Eren Nezaket, Altuntaş Yüksel, Öztürk Feyza Yener
Gazi University Faculty of Medicine, Medical Biochemistry, Ankara, Turkey.
Health Sciences University Ankara Education and Research Hospital, Medical Biochemistry, Ankara, Turkey.
J Med Biochem. 2019 Mar 26;38(4):481-488. doi: 10.2478/jomb-2019-0014. eCollection 2019 Oct.
The aim of this study is to examine the relationship among the changes in activities of paraoxonase (PON), arylesterase (ARE) and homocysteine thiolactonase (HTLase) enzyme having antioxidant properties and the development of diabetic nephropathy (DN), one of the most common complications of diabetes.
Normoalbuminuric type-2 diabetic patients (Group II, n=100), microalbuminuric type 2 diabetic patients (Group III, n=100) and the control group (Group I, n=100) were included in the study. The age and gender of the patient groups matched with the age and gender of the control group. HTLase, PON and ARE enzyme activities were measured by the spectrophotometric method using a g-thiobutyrinolactone, paraoxon, and phenylacetate substrates respectively. In this study, an autoanalyzer application was developed in order to measure HTLase enzyme activity for the first time.
Serum HTLase, ARE and PON activities of Group III and Group II were significantly low compared to HTLase, ARE and PON results of Group I (p<0.05).
Based on our results, PON, ARE and HTLase enzyme activities were found to be decreased due to the increase in the degree of DN.
本研究旨在探讨具有抗氧化特性的对氧磷酶（PON）、芳基酯酶（ARE）和同型半胱氨酸硫内酯酶（HTLase）的活性变化与糖尿病最常见并发症之一糖尿病肾病（DN）的发生发展之间的关系。
本研究纳入了正常白蛋白尿的2型糖尿病患者（II组，n = 100）、微量白蛋白尿的2型糖尿病患者（III组，n = 100）和对照组（I组，n = 100）。患者组的年龄和性别与对照组的年龄和性别相匹配。分别使用γ-硫代丁内酯、对氧磷和苯乙酸盐底物通过分光光度法测量HTLase、PON和ARE酶的活性。在本研究中，首次开发了一种自动分析仪应用程序来测量HTLase酶的活性。
与I组的HTLase、ARE和PON结果相比，III组和II组的血清HTLase、ARE和PON活性显著降低（p < 0.05）。
根据我们的结果，发现由于DN程度的增加，PON、ARE和HTLase酶的活性降低。
