Mrag Marwa, Kassab Asma, Omezzine Asma, Belkacem Chebil Raoua, Ben Fredj Ismail Fatma, Douki Nabiha, Laouani Kechrid Chedia, Bouslema Ali, Ben Amor Faten
University of Monastir, Faculty of Dental Medicine, Oral Health and Oro-Facial Rehabilitation Research Laboratory, Monastir, Tunisia.
University of Monastir, Faculty of Pharmacy, Monastir, Tunisia.
J Med Biochem. 2020 Jan 23;39(2):140-148. doi: 10.2478/jomb-2019-0019.
The purpose of the present study was to assess saliva reliability in diagnosis and monitoring type 2 diabetes instead of blood.
Blood and unstimulated whole saliva were collected from 300 type 2 diabetic subjects and 300 healthy controls in fasting. Then, the salivary flow rate was calculated. All parameters including glucose, urea, amylase, total protein, albumin, C-reactive protein (CRP), immunoglobulin A (IgA), potassium, calcium and chloride were assessed in the supernatant, using an autoanalyzer. Oral exam was conducted by a single examiner on full mouth excluding third molars. Statistical analysis was performed by the SPSS 20.0 version.
Saliva screening showed that glucose, urea, amylase, total protein, potassium, calcium and chloride were significantly higher in patients compared to controls (p < 0.05). Whereas, the IgA level and salivary flow rate were significantly reduced in patients (p < 0.05). No significant difference was found in albumin and CRP levels (p > 0.05). There was a significant positive correlation between salivary and plasma glucose levels (r = 0.887, and r = 0.900, p < 0.001), as well as, salivary and blood urea (r = 0.586, and r = 0.688, p < 0.001) in patients and controls, respectively.
From this study, saliva could be suggested as a useful diagnostic tool for type 2 diabetes.
本研究的目的是评估唾液在2型糖尿病诊断和监测中的可靠性，而非血液。
收集300名2型糖尿病患者和300名健康对照者空腹时的血液和非刺激性全唾液。然后计算唾液流速。使用自动分析仪对上清液中的所有参数进行评估，包括葡萄糖、尿素、淀粉酶、总蛋白、白蛋白、C反应蛋白（CRP）、免疫球蛋白A（IgA）、钾、钙和氯。由一名检查者对全口（不包括第三磨牙）进行口腔检查。使用SPSS 20.0版本进行统计分析。
唾液筛查显示，与对照组相比，患者的葡萄糖、尿素、淀粉酶、总蛋白、钾、钙和氯显著更高（p < 0.05）。而患者的IgA水平和唾液流速显著降低（p < 0.05）。白蛋白和CRP水平无显著差异（p > 0.05）。患者和对照组中，唾液和血浆葡萄糖水平之间存在显著正相关（r = 0.887和r = 0.900，p < 0.001），唾液和血液尿素之间也存在显著正相关（r = 0.586和r = 0.688，p < 0.001）。
从本研究来看，唾液可被认为是2型糖尿病的一种有用诊断工具。
