Klinger K W, Shuster C W, Klinger J
Eur J Clin Microbiol. 1985 Apr;4(2):201-6. doi: 10.1007/BF02013598.
Sera from 48 cystic fibrosis patients from two hospitals were screened for antibody against rods, non-toxic macromolecular structures which share antigenic determinants with Pseudomonas aeruginosa exotoxin A. A solid-phase radioimmunoassay employing (125I)-staphylococcal protein A was used to detect anti-rod IgG. Antibodies recognizing rods, exotoxin A, or both antigens, were demonstrated using a competitive radioimmunoassay in cystic fibrosis patient sera, and in sera from animals immunized with exotoxin A, rods, or infected with Pseudomonas aeruginosa. Anti-rod titers of cystic fibrosis patients (1.07 to 14 X control serum levels) inversely correlated with aggregate clinical evaluation scores, and in most instances, with X-ray scores. Since rods are non-toxic and cross-reactive with exotoxin A, they may represent therapeutically useful antigens for producing immunity to exotoxin A.
对来自两家医院的48名囊性纤维化患者的血清进行了筛查，以检测针对棒状体的抗体。棒状体是无毒的大分子结构，与铜绿假单胞菌外毒素A具有共同的抗原决定簇。采用（125I）-葡萄球菌蛋白A的固相放射免疫测定法检测抗棒状体IgG。使用竞争性放射免疫测定法在囊性纤维化患者血清以及用外毒素A、棒状体免疫或感染铜绿假单胞菌的动物血清中，证实了识别棒状体、外毒素A或两种抗原的抗体。囊性纤维化患者的抗棒状体滴度（为对照血清水平的1.07至14倍）与综合临床评估评分呈负相关，并且在大多数情况下，与X线评分也呈负相关。由于棒状体无毒且与外毒素A有交叉反应，它们可能是用于产生针对外毒素A免疫力的具有治疗作用的抗原。
