Department of Biochemistry and Environmental Chemistry, Institute of Biotechnology, The John Paul II Catholic University of Lublin, Konstantynów Street 1 I, 20-708 Lublin, Poland.
Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation-State Research Institute (IUNG-PIB), Czartoryskich Street 8, 24-100 Puławy, Poland.
Int J Mol Sci. 2019 Sep 8;20(18):4415. doi: 10.3390/ijms20184415.
Methanogenesis occurs in many natural environments and is used in biotechnology for biogas production. The efficiency of methane production depends on the microbiome structure that determines interspecies electron transfer. In this research, the microbial community retrieved from mining subsidence reservoir sediment was used to establish enrichment cultures on media containing different carbon sources (tryptone, yeast extract, acetate, CO/H). The microbiome composition and methane production rate of the cultures were screened as a function of the substrate and transition stage. The relationships between the microorganisms involved in methane formation were the major focus of this study. Methanogenic consortia were identified by next generation sequencing (NGS) and functional genes connected with organic matter transformation were predicted using the PICRUSt approach and annotated in the KEGG. The methane production rate (exceeding 12.8 mg CH L d) was highest in the culture grown with tryptone, yeast extract, and CO/H The analysis of communities that developed on various carbon sources casts new light on the ecophysiology of the recently described bacterial phylum and methanogenic representing the genera and . Furthermore, it is hypothesized that representatives of may support hydrogenotrophic methanogenesis.
产甲烷作用发生在许多自然环境中，并被用于生物技术中的沼气生产。甲烷生产的效率取决于微生物群落结构，而微生物群落结构决定了种间电子传递。在这项研究中，从采矿沉陷水库沉积物中回收的微生物群落被用于在含有不同碳源（蛋白胨、酵母提取物、乙酸盐、CO/H）的培养基上建立富集培养物。培养物的微生物群落组成和甲烷生成率作为底物和过渡阶段的函数进行筛选。本研究的主要重点是参与甲烷形成的微生物之间的关系。通过下一代测序（NGS）鉴定产甲烷菌群，并使用 PICRUSt 方法预测与有机质转化相关的功能基因，并在 KEGG 中进行注释。在以蛋白胨、酵母提取物和 CO/H 培养的培养物中，甲烷生成率（超过 12.8 mg CH 4 L -1 d -1）最高。对在各种碳源上发育的群落的分析为最近描述的细菌门和产甲烷菌的生态生理学提供了新的认识，代表了 和 属。此外，据推测， 属的代表可能支持氢营养型产甲烷作用。
