Next Generation Sequencing Department, BaseClear B.V., Sylviusweg 74, 2333 BE, Leiden, The Netherlands.
Bianomics Business Unit, BaseClear B.V., Sylviusweg 74, 2333 BE, Leiden, The Netherlands.
FEMS Microbiol Lett. 2018 Aug 1;365(16). doi: 10.1093/femsle/fny103.
Industrial biotechnology develops and applies microorganisms for the production of bioproducts and enzymes with applications ranging from food and feed ingredients and processing to bio-based chemicals, biofuels and pharmaceutical products. Next generation DNA sequencing technologies play an increasingly important role in improving and accelerating microbial strain development for existing and novel bio-products via screening, gene and pathway discovery, metabolic engineering and additional optimization and understanding of large-scale manufacturing. In this mini-review, we describe novel DNA sequencing and analysis technologies with a focus on applications to industrial strain development, enzyme discovery and microbial community analysis.
工业生物技术开发和应用微生物生产生物制品和酶，应用范围从食品和饲料成分及加工到基于生物的化学品、生物燃料和制药产品。下一代 DNA 测序技术在通过筛选、基因和途径发现、代谢工程以及对大规模制造的进一步优化和理解来改进和加速现有和新型生物产品的微生物菌株开发方面发挥着越来越重要的作用。在这篇综述中，我们描述了新型 DNA 测序和分析技术，重点介绍了它们在工业菌株开发、酶发现和微生物群落分析中的应用。
