Costa Joana P, Pinheiro M Joana F, Sousa Sílvia A, Botelho do Rego Ana M, Marques Fernanda, Oliveira M Conceição, Leitão Jorge H, P Mira Nuno, N N Carvalho M Fernanda
Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Instituto de Bioengenharia e Biociências, Departamento de Bioengenharia, Instituto Superior Técnico Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Antibiotics (Basel). 2019 Sep 10;8(3):144. doi: 10.3390/antibiotics8030144.
Hydroxide [Ag(OH)L] (L = L, L, L, L), oxide [{AgL}}(μ-O)] (L = L, L, L, L, L) or chloride [AgL]Cl, [Ag(L)]Cl complexes were obtained from reactions of mono- or bicamphorimine derivatives with Ag(OAc) or AgCl. The new complexes were characterized by spectroscopic (NMR, FTIR) and elemental analysis. X-ray photoelectron spectroscopy (XPS), ESI mass spectra and conductivity measurements were undertaken to corroborate formulations. The antimicrobial activity of complexes and some ligands were evaluated towards and , and strains of the bacterial species , , and based on the Minimum Inhibitory Concentrations (MIC). Complexes displayed very high activity against the species studied with the lowest MIC values (3.9 µg/mL) being observed for complexes and against . A significant feature of these redesigned complexes is their ability to sensitize , a trait that was not found for the previously investigated [Ag(NO)L] complexes. The MIC values of the complexes towards bacteria were in the range of those of [Ag(NO)L] and well above those of the precursors Ag(OAc) or AgCl. The activity of the complexes towards normal fibroblasts V79 was evaluated by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Results showed that the complexes have a significant cytotoxicity.
通过单樟脑亚胺衍生物或双樟脑亚胺衍生物与醋酸银或氯化银反应，得到了氢氧化物[Ag(OH)L]（L = L、L、L、L）、氧化物[{AgL}(μ - O)]（L = L、L、L、L、L）或氯化物[AgL]Cl、[Ag(L)]Cl配合物。通过光谱分析（核磁共振、傅里叶变换红外光谱）和元素分析对新配合物进行了表征。进行了X射线光电子能谱（XPS）、电喷雾电离质谱和电导率测量以证实其结构。基于最低抑菌浓度（MIC），评估了配合物和一些配体对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和白色念珠菌菌株的抗菌活性。配合物对所研究的菌种显示出非常高的活性，配合物和对白色念珠菌观察到最低的MIC值（3.9 μg/mL）。这些重新设计的配合物的一个显著特征是它们使增敏的能力，这是先前研究的[Ag(NO)L]配合物所没有的特性。配合物对细菌的MIC值在[Ag(NO)L]的范围内，且远高于前体醋酸银或氯化银的MIC值。通过MTT（3 - [4,5 - 二甲基噻唑 - 2 - 基] - 2,5 - 二苯基溴化四氮唑）试验评估了配合物对正常成纤维细胞V79的活性。结果表明配合物具有显著的细胞毒性。
