Division of Pharmacy and Optometry, Laboratory for Lipidomics and Lipid Biology, School of Heath Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United Kingdom.
Division of Musculoskeletal and Dermatological Sciences, School of Biological Sciences, Faculty of Biology, Medicine, and Health, The University of Manchester, Manchester, United Kingdom.
FASEB J. 2019 Nov;33(11):13014-13027. doi: 10.1096/fj.201901501R. Epub 2019 Sep 13.
Nutritional supplementation with fish oil or ω-3 (n-3) polyunsaturated fatty acids (PUFAs) has potential benefits for skin inflammation. Although the differential metabolism of the main n-3PUFA eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) could lead to distinct activities, there are no clinical studies comparing their relative efficacy in human skin. Following a 10-wk oral supplementation of healthy volunteers and using mass spectrometry-based lipidomics, we found that n-3PUFA mainly affected the epidermal mediator lipidome. EPA was more efficient than DHA in reducing production of arachidonic acid-derived lipids, and both n-3PUFA lowered -acyl ethanolamines. In UV radiation-challenged skin (3 times the minimum erythemal dose), EPA attenuated the production of proinflammatory lipids, whereas DHA abrogated the migration of Langerhans cells, as assessed by immunohistochemistry. Interestingly, n-3PUFA increased the infiltration of CD4 and CD8 T cells but did not alter the erythemal response, either the sunburn threshold or the resolution of erythema, as assessed by spectrophotometric hemoglobin index readings. As EPA and DHA differentially impact cutaneous inflammation through changes in the network of epidermal lipids and dendritic and infiltrating immune cells, they should be considered separately when designing interventions for cutaneous disease.-Kendall, A. C., Pilkington, S. M., Murphy, S. A., Del Carratore, F., Sunarwidhi, A. L., Kiezel-Tsugunova, M., Urquhart, P., Watson, R. E. B., Breitling, R., Rhodes, L. E., Nicolaou, A. Dynamics of the human skin mediator lipidome in response to dietary ω-3 fatty acid supplementation.
营养补充剂中的鱼油或 ω-3（n-3）多不饱和脂肪酸（PUFA）对皮肤炎症具有潜在益处。尽管主要的 n-3PUFA 二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的不同代谢可能导致不同的活性，但目前尚无临床研究比较它们在人体皮肤中的相对疗效。在对健康志愿者进行 10 周口服补充后，我们使用基于质谱的脂质组学发现，n-3PUFA 主要影响表皮介质脂质组。与 DHA 相比，EPA 更有效地减少花生四烯酸衍生脂质的产生，两种 n-3PUFA 均降低了酰基乙醇胺。在紫外线辐射（最小红斑剂量的 3 倍）下，EPA 可减轻促炎脂质的产生，而 DHA 则通过免疫组织化学评估可阻断朗格汉斯细胞的迁移。有趣的是，n-3PUFA 增加了 CD4 和 CD8 T 细胞的浸润，但对红斑反应既没有改变，也没有改变晒红阈值或红斑的消退，这通过分光光度计血红蛋白指数读数进行评估。由于 EPA 和 DHA 通过改变表皮脂质和树突状和浸润免疫细胞的网络来不同地影响皮肤炎症，因此在设计皮肤疾病干预措施时应分别考虑它们。-肯德尔，A.C.，皮尔金顿，S.M.，墨菲，S.A.，德尔卡瓦略，F.，苏纳威迪，A.L.，基泽尔-楚古诺娃，M.，厄夸尔，P.，沃森，R.E.B.，布赖特林，R.，罗兹，L.E.，尼古拉乌，A. 饮食 ω-3 脂肪酸补充对人类皮肤介质脂质组的动态影响。
