Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China.
Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Carbohydr Polym. 2019 Dec 1;225:115221. doi: 10.1016/j.carbpol.2019.115221. Epub 2019 Aug 20.
Alginate Oligosaccharide (AOS) is a natural biological carbohydrate extracted from seaweed. In our study, Arabidopsis thaliana was used to evaluate the AOS-induced resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Resistance was vitally enhanced at 25 mg/L in wild type (WT), showing the decreased disease index and bacteria colonies, burst of ROS and NO, high transcription expression of resistance genes PR1 and increased content of salicylic acid (SA). In SA deficient mutant (sid2), AOS-induced disease resistance dropped obviously compared to WT. The disease index was significantly higher than WT and the expression of recA and avrPtoB are two and four times lower than WT, implying that AOS induces disease resistance injecting Pst DC3000 after three days treatment by arousing the SA pathway. Our results provide a reference for the profound research and application of AOS in agriculture.
藻酸盐寡糖（AOS）是一种从海藻中提取的天然生物碳水化合物。在我们的研究中，拟南芥被用于评估 AOS 诱导对丁香假单胞菌 pv.番茄 DC3000（Pst DC3000）的抗性。在 25mg/L 时，野生型（WT）的抗性得到了极大的增强，表现为疾病指数和细菌菌落的减少、ROS 和 NO 的爆发、抗性基因 PR1 的转录表达增加和水杨酸（SA）含量的增加。在 SA 缺陷突变体（sid2）中，AOS 诱导的抗性明显低于 WT。疾病指数明显高于 WT，recA 和 avrPtoB 的表达水平分别比 WT 低 2 倍和 4 倍，这表明 AOS 通过激活 SA 途径在处理 3 天后注入 Pst DC3000 来诱导疾病抗性。我们的研究结果为 AOS 在农业中的深入研究和应用提供了参考。
