Yamaguchi Tetsuo, Ono Daisuke, Sato Ayami
Department of Microbiology and Infectious Disease, Toho University, Ota-ku, Tokyo, Japan.
Methods Mol Biol. 2020;2069:59-78. doi: 10.1007/978-1-4939-9849-4_4.
Methicillin-susceptible S. aureus changes to methicillin-resistant S. aureus (MRSA) upon the acquisition of staphylococcal cassette chromosome mec (SCCmec), a genomic island that encodes methicillin resistance. SCCmec elements in S. aureus are classified into different types based on the combination of mec gene complexes and ccr gene complexes, which share variations, five classes in mec and eight in ccr. To date, at least 13 types of SCCmec elements have been identified and each SCCmec type has individual characteristics. It is known that hospital-associated MRSA strains carry SCCmec elements of types, I, II, and III, and the majority of community-acquired MRSA strains carry characteristic SCCmec elements, type IV SCCmec or type V SCCmec. We herein describe multiplex PCR methods to type SCCmec elements by identifying the mec gene complex class and ccr gene complex type.
对甲氧西林敏感的金黄色葡萄球菌在获得葡萄球菌盒式染色体mec（SCCmec）后会转变为耐甲氧西林金黄色葡萄球菌（MRSA），SCCmec是一个编码甲氧西林耐药性的基因组岛。金黄色葡萄球菌中的SCCmec元件根据mec基因复合体和ccr基因复合体的组合分为不同类型，它们存在变异，mec有五类，ccr有八类。迄今为止，已鉴定出至少13种类型的SCCmec元件，且每种SCCmec类型都有各自的特点。已知医院相关的MRSA菌株携带I型、II型和III型的SCCmec元件，而大多数社区获得性MRSA菌株携带特征性的SCCmec元件，即IV型SCCmec或V型SCCmec。我们在此描述了通过鉴定mec基因复合体类别和ccr基因复合体类型来对SCCmec元件进行分型的多重PCR方法。
