Gao Jiejun, Lin Lu, Wei Alexander, Sepúlveda Maria S
Department of Forestry and Natural Resources and Bindley Biosciences Center, Purdue University, West Lafayette, Indiana, USA.
Department of Chemistry, Purdue University, West Lafayette, Indiana, USA.
Environ Sci Technol Lett. 2017 May 9;4(5):174-179. doi: 10.1021/acs.estlett.7b00074. Epub 2017 Apr 7.
Nanoparticles (NPs) in contact with biological fluids experience changes in surface chemistry that can impact their biodistribution and downstream physiological impact. One such change involves the formation of a protein corona (PC) on the surface of NPs. Here we present a foundational study on PC formation following the incubation of polyvinylpyrrolidone-coated AgNPs (PVP-AgNPs, 50 nm) in the plasma of smallmouth bass (). PC formation increases with exposure time and is also affected by gender, with AgNPs incubated in male plasma having slightly thinner PCs and less negative zeta potentials than those incubated in female plasma. Proteomic analysis also revealed gender-specific differences in PC composition: in particular, egg-specific proteins (vitellogenin (VTG) and zona pellucida (ZP) were identified only in PCs derived from female plasma, raising the possibility of their roles in AgNP-related reproductive toxicity by promoting their accumulation in developing oocytes.
与生物流体接触的纳米颗粒（NPs）会经历表面化学变化，这可能会影响它们的生物分布和下游生理影响。其中一种变化涉及在NPs表面形成蛋白质冠（PC）。在此，我们展示了一项基础研究，该研究是关于将聚乙烯吡咯烷酮包覆的银纳米颗粒（PVP-AgNPs，50纳米）在小口黑鲈的血浆中孵育后PC的形成情况。PC的形成随暴露时间增加，并且也受性别影响，与在雄性血浆中孵育的AgNPs相比，在雌性血浆中孵育的AgNPs所形成的PC稍薄且zeta电位更负。蛋白质组学分析还揭示了PC组成上的性别特异性差异：特别是，仅在源自雌性血浆的PC中鉴定出了卵特异性蛋白（卵黄蛋白原（VTG）和透明带（ZP）），这增加了它们通过促进其在发育中的卵母细胞中积累而在与AgNP相关的生殖毒性中发挥作用的可能性。
