Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, Egypt.
Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, 1 Alzeraa Street Postal Code 44511, Zagazig City, Sharkia Province, Egypt.
Fish Shellfish Immunol. 2019 Nov;94:427-433. doi: 10.1016/j.fsi.2019.09.033. Epub 2019 Sep 16.
Our study is considered to attempt reducing the immune-toxic and antioxidant impacts of exposure to fipronil (FP) on Nile tilapia, Oreochromis niloticus using the β-glucan (βG). Two hundred and seventy fingerlings of Nile tilapia were divided randomly into six groups (45 tilapias of each, in 3 replicates): group I control (CT) group nourished on a basal diet. Group II (βG) nourished a basal diet supplemented with 0.4% βG. Groups III (1/20 FP) and V (1/10 FP) was exposed to 1/20 and 1/10 of the 96 h LC of FP in water and nourished the basal diet respectively. Groups IV (1/20 FP+ βG) and VI (1/10 FP+ βG) were exposed to 1/20 and 1/10 FP concomitantly with 0.4% βG supplementation for 90 successive days. Growth performance metrics were higher in βG group than CT. While those metrics were fallen at exposure to 1/20 or 1/10 FP. Supplementation with βG elevated the IgM and lysozyme levels.Whereas, tilapias exposed to FP only at different concentration showed lowering of those compared to CT. Supplementation with βG was effectively augmented IgM and lysozyme in 1/20 FP exposed tilapias. Furthermore, in a minor grade at 1/10 FP exposed tilapias. Exposure to FP increased the activities of hepatic markers chiefly at 1/10, however the βG supplementation was successfully improved these markers. There was imbalance of cortisol level at FP exposure where, βG combining to FP alleviate this disparity. There was fallen in LDH, MDH and FDPase in βG tilapias where continuing raise in 1/10 FP followed by 1/20 FP. βG supplementation raise the level of GSH, without significant variations in MDA conversely occurs in FP alone. Genes expression of βG caused raise of both GPx and GR, without fluctuations in CAT and SOD. Exposure to FP diminishes all evaluated antioxidant genes. It could fulfilled that supplementation with βG successfully alleviated the immune-toxic and antioxidant impact of FP in tilapias.
我们的研究旨在通过β-葡聚糖（βG）来降低氟虫腈（FP）暴露对尼罗罗非鱼（Oreochromis niloticus）的免疫毒性和抗氧化影响。将 270 尾尼罗罗非鱼随机分为六组（每组 45 尾，3 个重复）：I 组对照组（CT），摄食基础日粮；II 组（βG），摄食添加 0.4%βG 的基础日粮；III 组（1/20 FP）和 V 组（1/10 FP）分别在水中暴露于 FP 的 96 h LC 的 1/20 和 1/10，并摄食基础日粮；IV 组（1/20 FP+βG）和 VI 组（1/10 FP+βG）同时暴露于 1/20 和 1/10 FP 并添加 0.4%βG 共 90 天。βG 组的生长性能指标高于 CT 组，而暴露于 1/20 或 1/10 FP 后，这些指标下降。βG 的添加提高了 IgM 和溶菌酶水平，而仅暴露于 FP 的罗非鱼则表现出比 CT 更低的水平。βG 的添加有效地提高了 1/20 FP 暴露的罗非鱼的 IgM 和溶菌酶水平。此外，在 1/10 FP 暴露的罗非鱼中，这一效果略有增强。FP 的暴露主要在 1/10 时增加了肝标志物的活性，然而，βG 的添加成功地改善了这些标志物。FP 暴露导致皮质醇水平失衡，而βG 与 FP 结合则减轻了这种差异。βG 组的 LDH、MDH 和 FDPase 水平下降，而 1/10 FP 继续升高后，1/20 FP 又继续升高。βG 的添加提高了 GSH 水平，而 MDA 则没有显著变化，而 FP 单独使用则相反。βG 对 GPx 和 GR 的基因表达都有促进作用，而对 CAT 和 SOD 没有波动。FP 的暴露降低了所有评估的抗氧化基因。可以得出结论，βG 的添加成功地减轻了 FP 对罗非鱼的免疫毒性和抗氧化影响。
