Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, UCB Lyon 1, ENS de Lyon, INRA, CNRS, 46 Allée d'Italie, 69364 Lyon Cedex 07, France.
Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, UCB Lyon 1, ENS de Lyon, INRA, CNRS, 46 Allée d'Italie, 69364 Lyon Cedex 07, France
Development. 2019 Oct 25;146(20):dev173377. doi: 10.1242/dev.173377.
Although accumulating evidence suggests that gene regulation is highly stochastic, genetic screens have successfully uncovered master developmental regulators, questioning the relationship between transcriptional noise and intrinsic robustness of development. To identify developmental modules that are more or less resilient to large-scale genetic perturbations, we used the polymerase II-associated factor 1 complex (Paf1c) mutant , which is impaired in several RNA polymerase II-dependent transcriptional processes. We found that the control of flower termination was not as robust as classically pictured. In angiosperms, the floral female organs, called carpels, display determinate growth: their development requires the arrest of stem cell maintenance. In mutant flowers, carpels displayed a highly variable morphology, with different degrees of indeterminacy defects up to wild-type size inflorescence emerging from carpels. This phenotype was associated with variable expression of two key regulators of flower termination and stem cell maintenance in flowers, and The phenotype was also dependent on growth conditions. Together, these results highlight the surprisingly plastic nature of stem cell maintenance in plants and its dependence on Paf1c.
尽管越来越多的证据表明基因调控具有高度随机性，但遗传筛选已经成功地发现了主要的发育调节剂，这对转录噪声与发育内在稳健性之间的关系提出了质疑。为了确定对大规模遗传扰动具有较大或较小弹性的发育模块，我们使用了聚合酶 II 相关因子 1 复合物（Paf1c）突变体，该突变体在几个 RNA 聚合酶 II 依赖的转录过程中受到损害。我们发现，花终止的控制并不像经典描述的那样稳健。在被子植物中，称为心皮的花雌性器官表现出确定的生长：它们的发育需要停止干细胞维持。在突变体花中，心皮表现出高度可变的形态，不定性缺陷的程度不同，直到从心皮中出现野生型大小的花序。这种表型与花终止和干细胞维持的两个关键调节剂的可变表达有关， 和 该表型也依赖于生长条件。总之，这些结果突出了植物中干细胞维持的惊人可塑性及其对 Paf1c 的依赖性。
