Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCBL, INRAE, CNRS , Lyon 69364 CEDEX 07, France.
Vietnam Academy of Science and Technology (VAST), University of Science and Technology of Hanoi , Ha Noi, Vietnam.
Biol Lett. 2024 May;20(5):20240099. doi: 10.1098/rsbl.2024.0099. Epub 2024 May 29.
How organisms produce organs with robust shapes and sizes is still an open question. In recent years, the sepal has been used as a model system to study this question because of its highly reproducible shape and size. One interesting aspect of the sepal is that its epidermis contains cells of very different sizes. Previous reports have qualitatively shown that sepals with more or less giant cells exhibit comparable final size and shape. Here, we investigate this question using quantitative approaches. We find that a mixed population of cell size modestly contribute to the normal width of the sepal but is not essential for its shape robustness. Furthermore, in a mutant with increased cell and organ growth variability, the change in final sepal shape caused by giant cells is exaggerated but the shape robustness is not affected. This formally demonstrates that sepal shape variability is robust to cell size heterogeneity.
生物体如何产生具有稳健形状和大小的器官仍然是一个悬而未决的问题。近年来，由于其高度可重复的形状和大小，萼片已被用作研究这个问题的模型系统。萼片的一个有趣方面是，其表皮包含大小非常不同的细胞。以前的报告已经定性地表明，具有或多或少巨型细胞的萼片表现出可比的最终大小和形状。在这里，我们使用定量方法研究这个问题。我们发现，细胞大小的混合群体适度有助于萼片的正常宽度，但对其形状稳健性不是必需的。此外，在细胞和器官生长变异性增加的突变体中，巨型细胞引起的最终萼片形状的变化被夸大，但形状稳健性不受影响。这正式证明了萼片形状的可变性对细胞大小的异质性具有稳健性。
