Lung Diseases and Allergy Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Pediatrics, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
J Clin Lab Anal. 2020 Jan;34(1):e23039. doi: 10.1002/jcla.23039. Epub 2019 Sep 20.
Vitamin D (Vit D) function in asthma progression has been studied well. The effects of genetic variations in Vit D pathway molecules have been also studied, although the results are contradicted. In the present study, for the first time we examined the Vit D pathway molecules included serum Vit D and vitamin D-binding protein (VDBP) and also genetic variations in the vitamin D receptor (VDR) and VDBP in a Kurdish population with asthma.
An enzyme-linked immunosorbent assay (ELISA) method was used to measure the serum Vit D and VDBP. VDR rs1544410 and rs2228570 and VDBP rs7041 were assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
The serum level of Vit D significantly decreased in asthmatic patients versus controls (16.26 ± 6.76 vs 23.05 ± 10.57 ng/mL, P value = .001). We observed an indirect correlation between Vit D and clinical findings. We also found an increased level of serum VDBP in patients as compared to the controls (1044.6 ± 310.82 vs 545.95 ± 121.73 µg/mL, P value < .0001). Besides, the risk of asthma progression was increased in patients with the VDR rs2228570 CC and VDBP rs7041 GG genotypes (OR = 3.56, P = .0382 and OR = 2.58, P = .01, respectively).
In summary, our results explain the influence of the genetic variations in VDR and VDBP in addition to Vit D and VDBP serum concentrations on asthma susceptibility in the Kurdish population.
维生素 D（Vit D）在哮喘进展中的作用已得到充分研究。维生素 D 途径分子的遗传变异的影响也已经过研究，尽管结果存在矛盾。在本研究中，我们首次在库尔德哮喘人群中检查了 Vit D 途径分子，包括血清 Vit D 和维生素 D 结合蛋白（VDBP），以及维生素 D 受体（VDR）和 VDBP 中的遗传变异。
采用酶联免疫吸附试验（ELISA）法测定血清 Vit D 和 VDBP。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测 VDR rs1544410 和 rs2228570 以及 VDBP rs7041。
与对照组相比，哮喘患者的血清 Vit D 水平显著降低（16.26±6.76 与 23.05±10.57 ng/mL，P 值=0.001）。我们观察到 Vit D 与临床发现之间存在间接相关性。我们还发现与对照组相比，患者血清 VDBP 水平升高（1044.6±310.82 与 545.95±121.73 µg/mL，P 值<0.0001）。此外，VDR rs2228570 CC 和 VDBP rs7041 GG 基因型患者哮喘进展的风险增加（OR=3.56，P=0.0382 和 OR=2.58，P=0.01）。
总之，我们的结果解释了 VDR 和 VDBP 中的遗传变异以及 Vit D 和 VDBP 血清浓度对库尔德人群哮喘易感性的影响。
