基于分子网络的策略，从延龄草中发现多乙酰基 18-降螺甾烷醇皂苷。
Molecular networking-based strategy for the discovery of polyacetylated 18-norspirostanol saponins from Trillium tschonoskii maxim.
机构信息
State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
出版信息
Phytochemistry. 2019 Dec;168:112125. doi: 10.1016/j.phytochem.2019.112125. Epub 2019 Sep 18.
Nine undescribed polyacetylated 18-norspirostanol saponins, trilliumosides A‒J, were obtained after a guidance based on a molecular networking strategy from the rhizomes of Trillium tschonoskii. Their structures were established by analysis of comprehensive spectroscopic data and chemical methods after their isolation in pure form. All isolated saponins were evaluated for their cytotoxicities against five selected human cancer cell lines (Huh7,A549,MCF-7,HepG2, and MOLT-4) and anti-inflammatory effects on a lipopolysaccharide (LPS)-stimulated NO production model in RAW264.7 macrophages. Trilliumoside D showed significant cytotoxicity against MOLT-4 cell lines with an IC value of 1.0 ± 0.1 μM, whereas trilliumosides H and I displayed remarkable anti-inflammatory effects on NO production with inhibitory rates of 56.3 ± 1.5 and 56.2 ± 2.2% at the concentration of 1.0 μM, respectively.
从延龄草（Trillium tschonoskii）根茎中，基于分子网络策略，获得了 9 种未经描述的聚乙酰化 18-降螺甾烷醇皂苷，分别为延龄草皂苷 A-J。通过全面的光谱数据分析和化学方法分离出纯化合物后，建立了它们的结构。所有分离得到的皂苷均对五种人癌细胞系（Huh7、A549、MCF-7、HepG2 和 MOLT-4）的细胞毒性进行了评估，并对 LPS 刺激 RAW264.7 巨噬细胞中 NO 产生模型的抗炎作用进行了评估。延龄草皂苷 D 对 MOLT-4 细胞系表现出显著的细胞毒性，IC 值为 1.0±0.1μM，而延龄草皂苷 H 和 I 对 NO 产生具有显著的抗炎作用，在 1.0μM 浓度下的抑制率分别为 56.3±1.5%和 56.2±2.2%。
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