Li Juncheng, Huang Xiaoling, Huang Hao, Huo Heqiang, Nguyen Chi D, Pian Ruiqi, Li Huaqiang, Ouyang Kunxi, Chen Xiaoyang
Guangdong Key Laboratory for Innovative Development and Utilization of ForestPlant Germplasm, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou, 510642, China.
Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
AMB Express. 2019 Sep 21;9(1):152. doi: 10.1186/s13568-019-0860-z.
Neolamarckia cadamba is an important fast growing tree species used for pulping and wood material in industry for it's desirable wood properties. As one of the most important content in wood, lignin provides structural integrity, strength, and hydrophobicity to the thickened cell walls and is the major factor contributing to biomass recalcitrance. It does not reduce the palatability of forage grass for animals, but it hinders the isolation of cellulose fibers and the efficient enzymatic depolymerization of cellulose and hemicellulose into fermentable sugars in biorefining processes by limiting the access by hydrolytic enzymes to their polysaccharide substrates. This work focused on analyzing the functions of NcCSE (Caffeoyl Shikimate Esterase, GenBank accession number: MG739672) and NcHCT (Hydroxycinnamoyl Transferase,GenBank accession number: MG739673) in the lignin biosynthetic process in order to improve the potential for utilization of leaves and wood from N. cadamba. The mutant phenotype of cse-2 was dramatically complemented to WT in the stable transgenic lines cse-35S::NcCSE, but overexpression of NcHCT in the cse-2 mutant did not have the same result as cse-35S::NcCSE, providing only partial complementation.
团花树是一种重要的速生树种，因其理想的木材特性而被用于工业制浆和木材加工。作为木材中最重要的成分之一，木质素为增厚的细胞壁提供结构完整性、强度和疏水性，并且是导致生物质顽固性的主要因素。它不会降低饲草对动物的适口性，但在生物精炼过程中，它会限制水解酶与多糖底物的接触，从而阻碍纤维素纤维的分离以及纤维素和半纤维素高效酶解聚为可发酵糖。这项工作着重分析团花树咖啡酰莽草酸酯酶（NcCSE，基因库登录号：MG739672）和羟基肉桂酰转移酶（NcHCT，基因库登录号：MG739673）在木质素生物合成过程中的功能，以提高团花树树叶和木材的利用潜力。在稳定转基因株系cse-35S::NcCSE中，cse-2的突变表型显著恢复到野生型，但在cse-2突变体中过表达NcHCT并没有产生与cse-35S::NcCSE相同的结果，仅提供了部分互补。
