Manawasinghe Ishara S, Dissanayake Asha J, Li Xinghong, Liu Mei, Wanasinghe Dhanushka N, Xu Jianping, Zhao Wensheng, Zhang Wei, Zhou Yueyan, Hyde Kevin D, Brooks Siraprapa, Yan Jiye
Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Center of Excellence in Fungal Research, Mae Fah Luang University, Mueang Chiang Rai, Thailand.
Front Microbiol. 2019 Sep 2;10:1936. doi: 10.3389/fmicb.2019.01936. eCollection 2019.
Grapevine trunk diseases have become one of the main threats to grape production worldwide, with species as an emerging group of pathogens in China. At present, relatively little is known about the taxonomy and genetic diversity of Chinese populations, including their relationships to other populations worldwide. Here, we conducted an extensive field survey in six provinces in China to identify and characterize species in grape vineyards. Ninety-four isolates were identified and analyzed using multi-locus phylogeny. The isolates belonged to eight species, including three novel taxa, , and three new host records, , and . The most commonly isolated species was . In addition, high genetic diversity was observed for in Chinese vineyards. Haplotype network analysis of isolates from China and Europe showed a close relationship between samples from the two geographical locations and evidence for recombination. In comparative pathogenicity testing, was the most aggressive taxon, whereas was the least aggressive. This study provides new insights into the species associated with grapevines in China, and our results can be used to develop effective disease management strategies.
葡萄树干病害已成为全球葡萄生产的主要威胁之一，在中国作为一类新兴的病原菌。目前，对于中国种群的分类学和遗传多样性，包括它们与全球其他种群的关系，了解相对较少。在此，我们在中国六个省份进行了广泛的田间调查，以鉴定和表征葡萄园中的物种。利用多位点系统发育分析鉴定并分析了94个分离株。这些分离株属于8个物种，包括3个新分类单元，以及3个新的寄主记录，和。最常分离到的物种是。此外，在中国葡萄园观察到了较高的遗传多样性。对来自中国和欧洲的分离株进行单倍型网络分析，结果表明这两个地理位置的样本之间关系密切，且存在重组证据。在比较致病性测试中，是最具侵袭性的分类单元，而侵袭性最小。本研究为中国与葡萄相关的物种提供了新的见解，我们的结果可用于制定有效的病害管理策略。
