State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China.
State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China.
Mater Sci Eng C Mater Biol Appl. 2019 Dec;105:110155. doi: 10.1016/j.msec.2019.110155. Epub 2019 Sep 1.
In this study, rubidium-containing mesoporous bioglass (Rb-MBG) scaffolds were formed with the investigation of the influence of Rb addition on angiogenic and osteogenic differentiation abilities of hBMSC. The phase composition, microstructure, pore size distribution, ion release, biological activity, drug loading rate, and release rate of Rb-MBG were characterized. The proliferation and differentiation of hBMSC, the markers of bone formation (ALP, COL-1) and angiogenesis (VEGF, HIF-1α), and wnt/β-catenin related-signaling pathway gene were studied by cell culture. Rb-MBG loaded with antibacterial agents enoxacin (ENX), coliforms and Staphylococcus aureus were cultured together to study the antibacterial effects. The results indicate that the samples have a 350-550 μm large pore structure and 4.5-5.5 nm mesoporous size. Adding Rb can increase the activity of ALP, the secretion of VEGF and COLI, and the expression of HIF-1α of hBMSCs. Rb containing MBG is likely to enhance the proliferation and differentiation of hBMSCs through the influence of Wnt/ß-catenin signal path. Rb-MBG scaffold can load effectively and release Rb ions and ENX continuously to damage the bacterial cell membrane with the synergistic effect, and therefore achieve antibacterial results. In conclusion, adding Rb to MBG supports angiogenesis and osteogenesis of hBMSCs, as well as antibacterial activity.
在这项研究中，形成了含铷介孔生物玻璃（Rb-MBG）支架，研究了 Rb 加入对 hBMSC 血管生成和成骨分化能力的影响。对 Rb-MBG 的相组成、微观结构、孔径分布、离子释放、生物活性、药物负载率和释放率进行了表征。通过细胞培养研究了 hBMSC 的增殖和分化、成骨标志物（ALP、COL-1）和血管生成标志物（VEGF、HIF-1α）以及 wnt/β-catenin 相关信号通路基因。将载有抗菌剂恩诺沙星（ENX）、大肠埃希菌和金黄色葡萄球菌的 Rb-MBG 进行培养，研究其抗菌效果。结果表明，样品具有 350-550μm 的大孔结构和 4.5-5.5nm 的介孔尺寸。添加 Rb 可以提高 hBMSCs 的 ALP 活性、VEGF 和 COLI 的分泌以及 HIF-1α的表达。含 Rb 的 MBG 可能通过 Wnt/β-catenin 信号通路影响增强 hBMSCs 的增殖和分化。Rb-MBG 支架能够有效负载并持续释放 Rb 离子和 ENX，通过协同作用破坏细菌细胞膜，从而达到抗菌效果。总之，在 MBG 中添加 Rb 可以支持 hBMSC 的血管生成和成骨作用，并具有抗菌活性。
