College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
Xiamen Engineering and Technological Research Center for Comprehensive Utilization of Marine Biological Resources, Xiamen 361021, China.
Mar Drugs. 2019 Sep 20;17(10):541. doi: 10.3390/md17100541.
Type 2 diabetes mellitus (T2DM) accounts for more than 90% of cases of diabetes mellitus, which is harmful to human health. Herein, neoagarooligosaccharides (NAOs) were prepared and their potential as a treatment of T2DM was evaluated in KunMing (KM) mice. Specifically, a T2DM mice model was established by the combination of a high-fat diet (HFD) and alloxan injection. Consequently, the mice were given different doses of NAOs (100, 200, or 400 mg/kg) and the differences among groups of mice were recorded. As a result of the NAOs treatment, the fasting blood glucose (FBG) was lowered and the glucose tolerance was improved as compared with the model group. As indicated by the immunohistochemistry assay, the NAOs treatment was able to ameliorate hepatic macrovesicular steatosis and hepatocyte swelling, while it also recovered the number of pancreatic β-cells. Additionally, NAOs administration benefited the antioxidative capacity in mice as evidenced by the upregulation of both glutathione peroxidase and superoxide dismutase activity and the significant reduction of the malondialdehyde concentration. Furthermore, NAOs, as presented by Western blotting, increased the expression of p-ERK1/2, p-JNK, NQO1, HO-1, and PPARγ, via the MAPK, Nrf2, and PPARγ signaling pathways, respectively. In conclusion, NAOs can be used to treat some complications caused by T2DM, and are beneficial in controlling the level of blood glucose and ameliorating the damage of the liver and pancreatic islands.
2 型糖尿病（T2DM）占糖尿病病例的 90%以上，对人类健康有害。在此，我们制备了 neoagarooligosaccharides（NAOs），并在昆明（KM）小鼠中评估了它们治疗 T2DM 的潜力。具体来说，通过高脂肪饮食（HFD）和丙烯醛注射相结合的方法建立 T2DM 小鼠模型。然后，给不同剂量的 NAOs（100、200 或 400mg/kg）处理小鼠，并记录各组小鼠的差异。NAOs 治疗降低了空腹血糖（FBG），改善了葡萄糖耐量，与模型组相比有显著差异。免疫组织化学检测结果表明，NAOs 治疗能够改善肝大泡脂肪变性和肝细胞肿胀，同时恢复胰岛 β 细胞数量。此外，NAOs 处理通过上调谷胱甘肽过氧化物酶和超氧化物歧化酶的活性以及显著降低丙二醛浓度，有益于小鼠的抗氧化能力。此外，Western blot 结果表明，NAOs 通过 MAPK、Nrf2 和 PPARγ 信号通路分别增加了 p-ERK1/2、p-JNK、NQO1、HO-1 和 PPARγ 的表达。综上所述，NAOs 可用于治疗 T2DM 引起的一些并发症，并有助于控制血糖水平，改善肝脏和胰岛损伤。
