Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, Beijing 100191, China.
Nutrients. 2017 Sep 26;9(10):1061. doi: 10.3390/nu9101061.
To determine whether treatment with ginseng oligopeptides (GOPs) could modulate hyperglycemia related to type 2 diabetes mellitus (T2DM) in rats induced by high-fat diet and low doses of alloxan, type 2 diabetes was induced in male Sprague-Dawley (SD) rats by injecting them once with 105 mg/kg alloxan and feeding them high-carbohydrate/high-fat diet with or without GOP administration (0.125, 0.5, and 2.0 g/kg Body Weight) for 7, 24, and 52 weeks. Oral glucose test tolerance (OGTT), plasma glucose, serum insulin, level of antioxidant, and beta cell function were measured. Morphological observation and immunohistochemistry study of insulin of islets was performed by light microscopy. The insulin level and the expression of NF-κB and Bcl-2 family in pancreatic islets were also detected by Western blot analysis. In addition, survival time and survival rate were observed. After the treatment, the abnormal OGTT were partially reversed by GOPs treatment in diabetic rats. The efficacy of GOPs was manifested in the amelioration of pancreatic damage, as determined by microscopy analysis. Moreover, GOPs treatment increased the normal insulin content and decreased the expression of the NF-κB-signaling pathway. Compared with those in the control model, the survival time and rate were significantly longer. It is suggested that GOPs exhibit auxiliary therapeutic potential for diabetes.
为了确定人参寡肽（GOPs）是否可以调节由高脂肪饮食和低剂量链脲佐菌素诱导的 2 型糖尿病（T2DM）大鼠的高血糖症，通过向雄性 Sprague-Dawley（SD）大鼠单次注射 105mg/kg 链脲佐菌素，并给予高碳水化合物/高脂肪饮食，或给予 GOP 治疗（0.125、0.5 和 2.0g/kg 体重），诱导 2 型糖尿病 7、24 和 52 周。测量口服葡萄糖耐量试验（OGTT）、血浆葡萄糖、血清胰岛素、抗氧化水平和β细胞功能。通过光镜对胰岛的形态观察和胰岛素免疫组织化学研究。通过 Western blot 分析还检测了胰岛中胰岛素水平以及 NF-κB 和 Bcl-2 家族的表达。此外，还观察了生存时间和生存率。治疗后，GOPs 治疗部分逆转了糖尿病大鼠的异常 OGTT。GOPs 的疗效表现为通过显微镜分析改善了胰腺损伤。此外，GOPs 治疗增加了正常的胰岛素含量并降低了 NF-κB 信号通路的表达。与对照组相比，生存时间和生存率明显更长。提示 GOPs 对糖尿病具有辅助治疗潜力。
