Yu Miao, Chambers James K, Tsuzuki Masano, Yamashita Nanako, Ushigusa Takahiro, Haga Takeshi, Nakayama Hiroyuki, Uchida Kazuyuki
Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Laboratory of Infection Control and Disease Prevention, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
J Vet Med Sci. 2019 Nov 29;81(11):1643-1648. doi: 10.1292/jvms.19-0384. Epub 2019 Sep 25.
Pigmented viral plaque is most commonly seen in Pug dogs in association with canine papillomavirus (CPV). In the present study, nucleic acid sequence and localization of viral genes were examined in 4 cases of pigmented viral plaque in Pug dogs. The results of polymerase chain reaction and nucleic acid sequence analysis showed that the 3 cases with pigmented viral plaque were infected with CPV4, and 1 case with CPV18. In the case with CPV18-positive viral plaque, CPV18 gene was also detected in a lesion of cytokeratin-14- and P63-positive basal cell tumor that developed adjacent to a pigmented viral plaque. Moreover, CPV gene was detected in the squamous cells of pigmented viral plaques and the neoplastic cells of basal cell tumor by in situ hybridization. This is the first report of basal cell tumor associated with CPV18-infection in the dog. Infection of CPV18 may be associated with development of basal cell tumor.
色素沉着性病毒斑块最常见于哈巴狗，与犬乳头瘤病毒（CPV）有关。在本研究中，对4例哈巴狗色素沉着性病毒斑块进行了病毒基因的核酸序列和定位检测。聚合酶链反应和核酸序列分析结果显示，3例色素沉着性病毒斑块感染了CPV4，1例感染了CPV18。在CPV18阳性病毒斑块的病例中，在与色素沉着性病毒斑块相邻发生的细胞角蛋白-14和P63阳性基底细胞瘤的病变中也检测到了CPV18基因。此外，通过原位杂交在色素沉着性病毒斑块的鳞状细胞和基底细胞瘤的肿瘤细胞中检测到了CPV基因。这是犬中与CPV18感染相关的基底细胞瘤的首次报道。CPV18感染可能与基底细胞瘤的发生有关。
