Li Hongbing, Hu Li, Zhang Huankang, Wang Dehui
Department of Otolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Department of Research Centre, Eye and ENT Hospital of Fudan University, Shanghai, China.
Acta Otolaryngol. 2019 Dec;139(12):1104-1111. doi: 10.1080/00016489.2019.1650200. Epub 2019 Sep 26.
Currently, the expression patterns of epidermal growth factor receptor (EGFR) family genes in sinonasal inverted papilloma (SNIP) and inverted papilloma with squamous cell carcinoma (IPwSCC) are not clear. This study aimed to investigate the expression of EGFR family members and their ligands in SNIP and IPwSCC and to analyze their correlations with SNIP histological grade and Krouse stage. Data from 25 cases of inverted papilloma patients in China were collected and divided into 16 cases in the SNIP group and 9 in the IPwSCC group. In addition, eight cases of normal nasal mucosa (NNM) were collected and used as the control group. The expression levels of EGFR family members and their ligands in the NNM and SNIP groups and EGFR family members in the IPwSCC group were evaluated using immunohistochemistry and qRT-PCR. In addition, their correlations with the SNIP histological grade and Krouse stage were analyzed. The statistical analysis was performed using the GraphPad Prism 7.0 statistical software. The ErbB1 and ErbB2 mRNA and protein expression levels were significantly higher in the SNIP group than in the NNM group ( < .01). The ErbB1 and ErbB2 protein expression levels were significantly higher in the IPwSCC group than those in the NNM and SNIP groups ( < .01). The ErbB1 and ErbB2 mRNA and protein expression levels in the SNIP group were positively correlated with the SNIP dysplasia grade. Upregulation of ErbB1 and ErbB2 expression may be associated with SNIP pathogenesis and carcinogenesis.
目前，表皮生长因子受体（EGFR）家族基因在鼻窦内翻性乳头状瘤（SNIP）和伴鳞状细胞癌的内翻性乳头状瘤（IPwSCC）中的表达模式尚不清楚。本研究旨在探讨EGFR家族成员及其配体在SNIP和IPwSCC中的表达，并分析它们与SNIP组织学分级和Krouse分期的相关性。收集了25例中国内翻性乳头状瘤患者的数据，分为SNIP组16例和IPwSCC组9例。此外，收集了8例正常鼻黏膜（NNM）作为对照组。采用免疫组织化学和qRT-PCR评估NNM组和SNIP组中EGFR家族成员及其配体的表达水平以及IPwSCC组中EGFR家族成员的表达水平。此外，分析了它们与SNIP组织学分级和Krouse分期的相关性。使用GraphPad Prism 7.0统计软件进行统计分析。SNIP组中ErbB1和ErbB2的mRNA和蛋白表达水平显著高于NNM组（<0.01）。IPwSCC组中ErbB1和ErbB2的蛋白表达水平显著高于NNM组和SNIP组（<0.01）。SNIP组中ErbB1和ErbB2的mRNA和蛋白表达水平与SNIP发育异常分级呈正相关。ErbB1和ErbB2表达上调可能与SNIP的发病机制和癌变有关。
