Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.
Laryngoscope. 2024 Sep;134(9):3941-3946. doi: 10.1002/lary.31416. Epub 2024 Mar 28.
Sinonasal inverted papilloma (SNIP) is a noncancerous tumor that develops in the mucous membrane of the nasal sinuses. Many malignancies are tightly linked to autophagy, an intracellular self-degradation mechanism. HMGB1 has demonstrated its ability to modulate autophagy in many pathological conditions. This work investigates how HMGB1 and other genes involved in autophagy contribute to SNIP.
The study included 45 patients with SNIP and a control group consisting of 28 individuals. In each group, qPCR was employed to examine the mRNA expression levels of genes correlated with autophagy and HMGB1. HMGB1 and genes associated with autophagy were examined for protein expression levels via Western Blot and immunohistochemical staining assays. At the same time, the association between HMGB1 and genes involved in autophagy was discovered through correlation analysis. Furthermore, Krouse staging was utilized for investigating the expression levels of HMGB1 and other autophagy-related genes at various stages in clinically staged SNIP patients.
LC3B, ATG5, and Beclin1 autophagy-related genes and HMGB1 were substantially expressed in SNIP. Additionally, there was a positive correlation between HMGB1 and these genes. During various phases of SNIP, the levels of HMGB1 expression and autophagy-related genes were notably elevated at stage T4 compared with stage T2.
Clinical staging in SNIP is correlated with HMGB1 expression in conjunction with autophagy-related genes LC3B, ATG5, and Beclin1, suggesting the possibility of novel prognostic indicators.
NA Laryngoscope, 134:3941-3946, 2024.
鼻窦内翻性乳头状瘤（SNIP）是一种发生在鼻腔鼻窦黏膜的非癌性肿瘤。许多恶性肿瘤与自噬密切相关，自噬是一种细胞内自我降解的机制。HMGB1 已经证明了它在许多病理条件下调节自噬的能力。这项工作研究了 HMGB1 以及其他参与自噬的基因如何促进 SNIP 的发生。
本研究纳入了 45 例 SNIP 患者和 28 例对照组。在每组中，通过 qPCR 检测与自噬和 HMGB1 相关的基因的 mRNA 表达水平。通过 Western Blot 和免疫组织化学染色检测 HMGB1 和与自噬相关的基因的蛋白表达水平。同时，通过相关性分析发现 HMGB1 与参与自噬的基因之间的关联。此外，利用 Krouse 分期研究 HMGB1 和其他自噬相关基因在临床分期 SNIP 患者不同阶段的表达水平。
LC3B、ATG5 和 Beclin1 自噬相关基因和 HMGB1 在 SNIP 中大量表达。此外，HMGB1 与这些基因之间存在正相关。在 SNIP 的各个阶段，与 T2 期相比，T4 期 HMGB1 表达和自噬相关基因的水平显著升高。
SNIP 的临床分期与 HMGB1 表达以及与自噬相关的基因 LC3B、ATG5 和 Beclin1 相关，提示存在新的预后指标的可能性。
无 喉镜，134：3941-3946，2024。
