Takehara T, Itoh M, Hanada N, Saeki E
J Dent Res. 1985 Mar;64(3):447-9. doi: 10.1177/00220345850640031101.
Streptococcus mutans alone or cell-free glucosyltransferase (G-Tase)-together with either Streptococcus sanguis, Streptococcus salivarius, Actinomyces viscosus, or Lactobacillus casei cells-formed artificial dental plaque that firmly adhered to glass electrodes in a continuous culture system containing sucrose. The pH in these artificial plaque samples decreased more than did that of the surrounding medium. In the absence of GTase, the bacteria other than S. mutans did not form firmly-adhering plaque on glass electrodes. The pH of the plaque formed with GTase alone did not show the pH decrease seen when the plaque contained bacteria, but, because it catalyzed the synthesis of glucan, it is suggested that the glucan acts as a diffusion barrier to retard acid loss from plaque containing acid-producing bacteria.
变形链球菌单独，或与无细胞葡糖基转移酶（G-Tase）一起，再与血链球菌、唾液链球菌、粘性放线菌或干酪乳杆菌细胞共同作用，在含有蔗糖的连续培养系统中形成了牢固粘附于玻璃电极上的人工牙菌斑。这些人工菌斑样本中的pH值下降幅度比周围培养基的pH值下降幅度更大。在没有葡糖基转移酶的情况下，除变形链球菌外的其他细菌不会在玻璃电极上形成牢固粘附的菌斑。仅由葡糖基转移酶形成的菌斑的pH值并未呈现出含有细菌的菌斑所出现的pH值下降情况，但是，由于它催化了葡聚糖的合成，因此有人提出，葡聚糖起到了扩散屏障的作用，以减缓产酸细菌形成的菌斑中的酸流失。
