Single Molecule Biophysics, Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, 60438 Frankfurt, Germany.
Nanoscale. 2019 Oct 10;11(39):17981-17991. doi: 10.1039/c9nr06364a.
Optical super-resolution microscopy has revolutionized our understanding of cell biology. Next to visualizing cellular structures with near-molecular spatial resolution, an additional benefit is the molecular characterization of biomolecular complexes directly in an intact cell. Single-molecule localization microscopy, as one technology out of the toolbox of super-resolution methods, generates images by detecting the position of single fluorophore labels and is particularly suited for molecular quantification. We review imaging and analysis methods employing single-molecule localization microscopy and extract molecule numbers.
光学超分辨率显微镜极大地改变了我们对细胞生物学的理解。除了以接近分子的空间分辨率观察细胞结构外，另一个好处是可以直接在完整细胞中对生物分子复合物进行分子表征。单分子定位显微镜作为超分辨率方法工具包中的一项技术，通过检测单个荧光团标记的位置来生成图像，特别适合于分子定量。我们回顾了采用单分子定位显微镜的成像和分析方法，并提取了分子数量。
