通过基于基因融合的通用方法分离和鉴定大肠杆菌δ ompB菌株。
Isolation and characterization of delta ompB strains of Escherichia coli by a general method based on gene fusions.
作者信息
Garrett S, Taylor R K, Silhavy T J, Berman M L
出版信息
J Bacteriol. 1985 May;162(2):840-4. doi: 10.1128/jb.162.2.840-844.1985.
Abstract
We isolated a series of delta ompR delta envZ mutants by inducing a strain carrying a lambda prophage in the closely linked gene malP and screening the bacterial survivors for loss of the major outer membrane porins OmpF and OmpC. Characterization of these deletion strains showed that both OmpR and EnvZ were necessary for transcription of ompF and ompC and that neither gene was essential for cell viability. Moreover, the deletion strains did not exhibit the pleiotropic membrane protein deficiency observed with certain envZ mutants. The method described should allow the simple isolation of deletions in any region of the chromosome.
摘要
我们通过诱导携带λ原噬菌体的菌株在紧密连锁基因malP中发生突变，并筛选细菌幸存者中主要外膜孔蛋白OmpF和OmpC的缺失情况，分离出了一系列ompR缺失和envZ缺失的突变体。对这些缺失菌株的表征表明，OmpR和EnvZ对于ompF和ompC的转录都是必需的，且这两个基因对于细胞活力都不是必需的。此外，这些缺失菌株并未表现出某些envZ突变体所观察到的多效性膜蛋白缺陷。所描述的方法应能实现染色体任何区域缺失的简单分离。
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