Liu Xianping, Du Chengjuan, Li Haichun, Jiang Ting, Luo Zimiao, Pang Zhiqing, Geng Daoying, Zhang Jun
Department of Radiology, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai 200040, China.
School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, 826 Zhangheng Road, Shanghai 201203, China.
Beilstein J Nanotechnol. 2019 Sep 11;10:1860-1872. doi: 10.3762/bjnano.10.181. eCollection 2019.
In this work, a peptide-modified, biodegradable, nontoxic, brain-tumor-targeting nanoprobe based on superparamagnetic iron oxide nanoparticles (SPIONs) (which have been commonly used as -weighted magnetic resonance (MR) contrast agents) was successfully synthesized and applied for accurate molecular MR imaging and sensitive optical imaging. PEPHC1, a short peptide which can specifically bind to epidermal growth factor receptor variant III (EGFRvIII) that is overexpressed in glioblastoma, was conjugated with SPIONs to construct the nanoprobe. Both in vitro and in vivo MR and optical imaging demonstrated that the as-constructed nanoprobe was effective and sensitive for tumor targeting with desirable biosafety. Given its desirable properties such as a 100 nm diameter (capable of penetration of the blood-brain barrier) and bimodal imaging capability, this novel and versatile multimodal nanoprobe could bring a new perspective for elucidating intracranial glioblastoma preoperative diagnosis and the accuracy of tumor resection.
在本研究中，基于超顺磁性氧化铁纳米颗粒（SPIONs）（其通常用作T2加权磁共振（MR）造影剂）成功合成了一种肽修饰的、可生物降解的、无毒的、脑肿瘤靶向纳米探针，并将其应用于精确的分子MR成像和灵敏的光学成像。将能特异性结合在胶质母细胞瘤中过表达的表皮生长因子受体变体III（EGFRvIII）的短肽PEPHC1与SPIONs偶联以构建纳米探针。体外和体内MR及光学成像均表明，所构建的纳米探针对于肿瘤靶向有效且灵敏，具有良好的生物安全性。鉴于其具有如100 nm直径（能够穿透血脑屏障）和双模态成像能力等理想特性，这种新型且多功能的多模态纳米探针可为阐明颅内胶质母细胞瘤的术前诊断及肿瘤切除的准确性带来新的视角。
