• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过细胞传代降低猪急性腹泻综合征冠状病毒强毒株的毒力。

Attenuation of a virulent swine acute diarrhea syndrome coronavirus strain via cell culture passage.

机构信息

College of Animal Science, South China Agricultural University, Guangzhou, China.

College of Animal Science, South China Agricultural University, Guangzhou, China.

出版信息

Virology. 2019 Dec;538:61-70. doi: 10.1016/j.virol.2019.09.009. Epub 2019 Sep 21.

DOI:10.1016/j.virol.2019.09.009
PMID:31580972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7112038/
Abstract

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly identified enteric alphacoronavirus that causes fatal diarrhea in newborn piglets in China. Here, we propagated a virulent strain SADS-CoV/CN/GDWT/2017 in Vero cells for up to 83 passages. Four strains of SADS-CoV/GDWT-P7, -P18, -P48 and -P83 were isolated and characterized. Sequence alignments showed that these four novel strains exhibited 16 nucleotide mutations and resultant 10 amino acid substitutions in open reading frame 1a/1b, spike, NS3a, envelope, membrane and nucleocapsid proteins. Furthermore, a 58-bp deletion in NS7a/7b was found in P48 and P83 strains, which led to the loss of NS7b and 38 amino acid changes of NS7a. Pig infection studies showed that the P7 strain caused typical watery diarrhea, while the P83 strain induced none-to-mild, delayed and transient diarrhea. This is the first report on cell adaption of a virulent SADS-CoV strain, which gives information on the potential virulence determinants of SADS-CoV.

摘要

猪急性腹泻综合征冠状病毒（SADS-CoV）是一种新鉴定的肠道α冠状病毒，可导致中国新生仔猪致命性腹泻。在此，我们在 Vero 细胞中传代培养了一株强毒 SADS-CoV/CN/GDWT/2017 株，达 83 代。我们分离并鉴定了 4 株 SADS-CoV/GDWT-P7、-P18、-P48 和 -P83。序列比对显示，这 4 株新型毒株在 1a/1b、刺突、NS3a、包膜、膜和核衣壳蛋白的开放阅读框中显示出 16 个核苷酸突变和由此导致的 10 个氨基酸替换。此外，在 P48 和 P83 株中发现 NS7a/7b 中有 58 个碱基缺失，导致 NS7b 缺失和 NS7a 38 个氨基酸改变。猪感染研究表明，P7 株引起典型水样腹泻，而 P83 株引起无至轻度、延迟和短暂性腹泻。这是首例关于强毒 SADS-CoV 株细胞适应的报道，为 SADS-CoV 的潜在毒力决定因素提供了信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/d293f5ff82d9/gr9_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/bcc7a2a30029/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/8bedc56918a2/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/ed9dd02a7efe/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/e347f6abd8ee/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/d35047cd9c75/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/503e4f3ea284/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/392f71315012/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/f107f4bea5a9/gr8_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/d293f5ff82d9/gr9_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/bcc7a2a30029/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/8bedc56918a2/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/ed9dd02a7efe/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/e347f6abd8ee/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/d35047cd9c75/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/503e4f3ea284/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/392f71315012/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/f107f4bea5a9/gr8_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abde/7112038/d293f5ff82d9/gr9_lrg.jpg

相似文献

1
Attenuation of a virulent swine acute diarrhea syndrome coronavirus strain via cell culture passage.通过细胞传代降低猪急性腹泻综合征冠状病毒强毒株的毒力。
Virology. 2019 Dec;538:61-70. doi: 10.1016/j.virol.2019.09.009. Epub 2019 Sep 21.
2
Characterization of a novel bat-HKU2-like swine enteric alphacoronavirus (SeACoV) infection in cultured cells and development of a SeACoV infectious clone.一种新型蝙蝠-HKU2 样猪肠道α冠状病毒（SeACoV）在细胞培养中的特征描述及 SeACoV 感染性克隆的建立。
Virology. 2019 Oct;536:110-118. doi: 10.1016/j.virol.2019.08.006. Epub 2019 Aug 9.
3
NS7a of SADS-CoV promotes viral infection via inducing apoptosis to suppress type III interferon production.SADS-CoV 的 NS7a 通过诱导细胞凋亡抑制 III 型干扰素的产生从而促进病毒感染。
J Virol. 2024 May 14;98(5):e0031724. doi: 10.1128/jvi.00317-24. Epub 2024 Apr 16.
4
Broad Cross-Species Infection of Cultured Cells by Bat HKU2-Related Swine Acute Diarrhea Syndrome Coronavirus and Identification of Its Replication in Murine Dendritic Cells Highlight Its Potential for Diverse Interspecies Transmission.蝙蝠 HKU2 相关猪急性腹泻综合征冠状病毒对培养细胞的广泛跨种感染及其在鼠树突状细胞中的复制鉴定提示其具有潜在的多种种间传播能力。
J Virol. 2019 Nov 26;93(24). doi: 10.1128/JVI.01448-19. Print 2019 Dec 15.
5
Attenuation and characterization of porcine enteric alphacoronavirus strain GDS04 via serial cell passage.通过连续细胞传代衰减和鉴定猪肠道α冠状病毒 GDS04 株。
Vet Microbiol. 2019 Dec;239:108489. doi: 10.1016/j.vetmic.2019.108489. Epub 2019 Nov 4.
6
The re-emerging of SADS-CoV infection in pig herds in Southern China.中国南方猪群中 SARS-CoV 感染的再现。
Transbound Emerg Dis. 2019 Sep;66(5):2180-2183. doi: 10.1111/tbed.13270. Epub 2019 Jul 10.
7
Epitope mapping and cellular localization of swine acute diarrhea syndrome coronavirus nucleocapsid protein using a novel monoclonal antibody.利用新型单克隆抗体进行猪急性腹泻综合征冠状病毒核衣壳蛋白的表位作图和细胞定位。
Virus Res. 2019 Nov;273:197752. doi: 10.1016/j.virusres.2019.197752. Epub 2019 Sep 10.
8
Re-emergence of severe acute diarrhea syndrome coronavirus (SADS-CoV) in Henan, central China, 2023.2023 年中国中部河南省严重急性腹泻综合征冠状病毒（SADS-CoV）再现。
Vet Microbiol. 2024 May;292:110049. doi: 10.1016/j.vetmic.2024.110049. Epub 2024 Mar 15.
9
Attenuation of an original US porcine epidemic diarrhea virus strain PC22A via serial cell culture passage.通过连续细胞培养传代对原始美国猪流行性腹泻病毒株PC22A进行减毒。
Vet Microbiol. 2017 Mar;201:62-71. doi: 10.1016/j.vetmic.2017.01.015. Epub 2017 Jan 15.
10
Comprehensive Subcellular Localization of Swine Acute Diarrhea Syndrome Coronavirus Proteins.猪流行性腹泻病毒蛋白的全面亚细胞定位。
J Virol. 2022 Sep 14;96(17):e0077222. doi: 10.1128/jvi.00772-22. Epub 2022 Aug 16.

引用本文的文献

1
Recombinant SADS-CoV as a vector for porcine epidemic diarrhea vaccine development.重组猪急性腹泻综合征冠状病毒作为猪流行性腹泻疫苗开发的载体。
Front Immunol. 2025 Aug 6;16:1633661. doi: 10.3389/fimmu.2025.1633661. eCollection 2025.
2
Swine Acute Diarrhea Syndrome Coronavirus: An Overview of Virus Structure and Virus-Host Interactions.猪急性腹泻综合征冠状病毒：病毒结构与病毒-宿主相互作用概述
Animals (Basel). 2025 Jan 9;15(2):149. doi: 10.3390/ani15020149.
3
Bat Rhinacoviruses Related to Swine Acute Diarrhoea Syndrome Coronavirus Evolve under Strong Host and Geographic Constraints in China and Vietnam.

本文引用的文献

1
The re-emerging of SADS-CoV infection in pig herds in Southern China.中国南方猪群中 SARS-CoV 感染的再现。
Transbound Emerg Dis. 2019 Sep;66(5):2180-2183. doi: 10.1111/tbed.13270. Epub 2019 Jul 10.
2
Recombinant vesicular stomatitis virus expressing the spike protein of genotype 2b porcine epidemic diarrhea virus: A platform for vaccine development against emerging epidemic isolates.表达 2b 基因型猪流行性腹泻病毒刺突蛋白的重组水疱性口炎病毒：针对新兴流行分离株的疫苗开发平台。
Virology. 2019 Jul;533:77-85. doi: 10.1016/j.virol.2019.05.009. Epub 2019 May 21.
3
Emerging and re-emerging coronaviruses in pigs.
中越两国受宿主和地理因素强烈影响的蝙蝠冠状病毒与猪急性腹泻综合征冠状病毒相关。
Viruses. 2024 Jul 11;16(7):1114. doi: 10.3390/v16071114.
4
Research Advances on Swine Acute Diarrhea Syndrome Coronavirus.猪急性腹泻综合征冠状病毒的研究进展
Animals (Basel). 2024 Jan 30;14(3):448. doi: 10.3390/ani14030448.
5
Prevention and Control of Swine Enteric Coronaviruses in China: A Review of Vaccine Development and Application.中国猪肠道冠状病毒的防控：疫苗研发与应用综述
Vaccines (Basel). 2023 Dec 21;12(1):11. doi: 10.3390/vaccines12010011.
6
Identification of a receptor tyrosine kinase inhibitor CP-724714 inhibits SADS-CoV related swine diarrhea coronaviruses infection in vitro.鉴定一种受体酪氨酸激酶抑制剂 CP-724714 可抑制 SADS-CoV 相关猪腹泻冠状病毒的体外感染。
Virol Sin. 2023 Oct;38(5):778-786. doi: 10.1016/j.virs.2023.06.010. Epub 2023 Jul 3.
7
Combined Analysis of the Whole Transcriptome of Piglets Infected with SADS-CoV Virulent and Avirulent Strains.感染SADS-CoV强毒株和无毒株仔猪全转录组的联合分析
Microorganisms. 2023 Feb 6;11(2):409. doi: 10.3390/microorganisms11020409.
8
Lethal Swine Acute Diarrhea Syndrome Coronavirus Infection in Suckling Mice.致死性猪急性腹泻综合征冠状病毒感染乳鼠。
J Virol. 2022 Sep 14;96(17):e0006522. doi: 10.1128/jvi.00065-22. Epub 2022 Aug 22.
9
Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids.胆汁酸促进猪急性腹泻综合征冠状病毒通过猪肠类器官的小窝相关内吞作用进入细胞。
PLoS Pathog. 2022 Jun 13;18(6):e1010620. doi: 10.1371/journal.ppat.1010620. eCollection 2022 Jun.
10
Swine Enteric Coronavirus: Diverse Pathogen-Host Interactions.猪传染性胃肠炎病毒：不同的病原体-宿主相互作用。
Int J Mol Sci. 2022 Apr 2;23(7):3953. doi: 10.3390/ijms23073953.
猪中新兴和再现的冠状病毒。
Curr Opin Virol. 2019 Feb;34:39-49. doi: 10.1016/j.coviro.2018.12.001. Epub 2019 Jan 14.
4
A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate.一株新分离的中国强毒基因型 GIIb 猪流行性腹泻病毒株：生物学特性、致病性及作为灭活疫苗候选株的免疫保护效果。
Virus Res. 2019 Jan 2;259:18-27. doi: 10.1016/j.virusres.2018.10.012. Epub 2018 Oct 17.
5
The Characterization of Immunoprotection Induced by a cDNA Clone Derived from the Attenuated Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strain.源自减毒台湾猪流行性腹泻病毒 Pintung 52 株 cDNA 克隆诱导的免疫保护特性。
Viruses. 2018 Oct 4;10(10):543. doi: 10.3390/v10100543.
6
Retrospective detection and phylogenetic analysis of swine acute diarrhoea syndrome coronavirus in pigs in southern China.中国南方猪群中猪急性腹泻综合征冠状病毒的回溯性检测与系统进化分析。
Transbound Emerg Dis. 2019 Mar;66(2):687-695. doi: 10.1111/tbed.13008. Epub 2019 Jan 9.
7
Isolation and characterization of a highly pathogenic strain of Porcine enteric alphacoronavirus causing watery diarrhoea and high mortality in newborn piglets.从导致新生仔猪水样腹泻和高死亡率的猪肠道α冠状病毒中分离和鉴定一株高致病性毒株。
Transbound Emerg Dis. 2019 Jan;66(1):119-130. doi: 10.1111/tbed.12992. Epub 2018 Sep 10.
8
Porcine epidemic diarrhea vaccine evaluation using a newly isolated strain from Korea.使用韩国新分离株评估猪流行性腹泻疫苗。
Vet Microbiol. 2018 Jul;221:19-26. doi: 10.1016/j.vetmic.2018.05.012. Epub 2018 May 24.
9
Newly emerged porcine enteric alphacoronavirus in southern China: Identification, origin and evolutionary history analysis.中国南方新出现的猪肠道α冠状病毒：鉴定、起源和进化史分析。
Infect Genet Evol. 2018 Aug;62:179-187. doi: 10.1016/j.meegid.2018.04.031. Epub 2018 Apr 25.
10
Fatal swine acute diarrhoea syndrome caused by an HKU2-related coronavirus of bat origin.由源自蝙蝠的 HKU2 相关冠状病毒引起的致命猪急性腹泻综合征。
Nature. 2018 Apr;556(7700):255-258. doi: 10.1038/s41586-018-0010-9. Epub 2018 Apr 4.