Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, Rome, Italy.
Institute for Systems Analysis and Computer Science "Antonio Ruberti", National Research Council, Rome, Italy.
Sci Rep. 2019 Oct 4;9(1):14350. doi: 10.1038/s41598-019-50575-5.
The variations in the membrane proteome of tomato fruit pericarp during ripening have been investigated by mass spectrometry-based label-free proteomics. Mature green (MG30) and red ripe (R45) stages were chosen because they are pivotal in the ripening process: MG30 corresponds to the end of cellular expansion, when fruit growth has stopped and fruit starts ripening, whereas R45 corresponds to the mature fruit. Protein patterns were markedly different: among the 1315 proteins identified with at least two unique peptides, 145 significantly varied in abundance in the process of fruit ripening. The subcellular and biochemical fractionation resulted in GO term enrichment for organelle proteins in our dataset, and allowed the detection of low-abundance proteins that were not detected in previous proteomic studies on tomato fruits. Functional annotation showed that the largest proportion of identified proteins were involved in cell wall metabolism, vesicle-mediated transport, hormone biosynthesis, secondary metabolism, lipid metabolism, protein synthesis and degradation, carbohydrate metabolic processes, signalling and response to stress.
采用基于质谱的无标记蛋白质组学方法研究了番茄果实果皮在成熟过程中膜蛋白质组的变化。选择成熟绿色（MG30）和红色成熟（R45）阶段是因为它们在成熟过程中是关键的：MG30 对应于细胞扩展的结束，此时果实生长停止，果实开始成熟，而 R45 对应于成熟果实。蛋白质图谱明显不同：在至少有两个独特肽段鉴定的 1315 种蛋白质中，有 145 种在果实成熟过程中的丰度显著变化。亚细胞和生化分级分离导致我们的数据集的细胞器蛋白质的 GO 术语富集，并允许检测到在以前的番茄果实蛋白质组学研究中未检测到的低丰度蛋白质。功能注释表明，鉴定出的蛋白质最大比例参与细胞壁代谢、囊泡介导的运输、激素生物合成、次生代谢、脂质代谢、蛋白质合成和降解、碳水化合物代谢过程、信号转导和应激反应。
