Samkoe Kimberley S, Sardar Hira Shahzad, Gunn Jason, Feldwisch Joachim, Linos Konstantinos, Henderson Eric, Pogue Brian, Paulsen Keith
Geisel School of Medicine, Dartmouth College, Hanover, NH 03755.
Department of Surgery, Dartmouth-Hitchcock, Lebanon, NH, 03756.
Proc SPIE Int Soc Opt Eng. 2019 Feb;10862. doi: 10.1117/12.2510935. Epub 2019 May 22.
Microdose administration of ABY-029, an anti-epidermal growth factor receptor Affibody molecule conjugated to IRDye 800CW, is being studied in a Phase 0 trial for resection of soft-tissue sarcomas. The excised tissue of a single patient in the microdose administration group was imaged with both a wide-field fluorescence surgical system and a flat-bed scanning fluorescence imaging system. Here the resultant fluorescence from a breadloaf section of the primary tumor specimen and six region-specific tissue samples collected from that breadloaf are compared using these two imaging systems - a flatbed, black-box, fluorescence scanning system, the Odyssey CLx, and a open-air, wide-field, pre-clinical surgical imaging system, the Solaris. Florescence signal is compared using a variety of methods including: mean, standard deviation, variance, tumor-to-background ratio, biological-variance ratio, and contrast-to-noise ratio. The images produced from the Odyssey scanner have higher signal variance but more accurately represent the EGFR expression in small tissue sections. The Solaris system has higher depth sensitivity and volume averaging, and as such has lower signal variation and higher contrast-to-noise ratio.
一种与IRDye 800CW偶联的抗表皮生长因子受体亲和分子ABY-029的微剂量给药，正在一项针对软组织肉瘤切除术的0期试验中进行研究。微剂量给药组中一名患者的切除组织，使用宽视野荧光手术系统和平板扫描荧光成像系统进行成像。在这里，使用这两种成像系统——平板式黑匣子荧光扫描系统Odyssey CLx和露天宽视野临床前手术成像系统Solaris，比较原发性肿瘤标本的面包片切片和从该面包片中收集的六个区域特异性组织样本所产生的荧光。使用多种方法比较荧光信号，包括：均值、标准差、方差、肿瘤与背景比值、生物方差比值和对比度噪声比值。Odyssey扫描仪生成的图像具有更高的信号方差，但更准确地反映了小组织切片中的表皮生长因子受体（EGFR）表达。Solaris系统具有更高的深度敏感性和体积平均效应，因此具有更低的信号变化和更高的对比度噪声比值。
