Hajialilo Elham, Hosseini-Safa Ahmad, Spotin Adel, Saraei Mehrzad, Ghanbari Johkool Morteza, Piri Hossein, Heydarian Peyman
Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Parasitology and Mycology, Qazvin University of Medical Sciences, Qazvin, Iran.
Iran J Public Health. 2023 Apr;52(4):818-828. doi: 10.18502/ijph.v52i4.12455.
The liver flukes of the species and spp. are recognised as parasites of domestic and wild herbivores. Both species of and as well as are distributed in Iran. The present study aimed to identify spp. and spp. using mitochondrial (cytochrome c oxidase I) gene by HRM method.
Totally, thirty infected liver specimens were collected from the sheep (n:23) and cattle (n:7) at the abattoirs of Qazvin Province, northwest Iran in 2022. DNA extraction and PCR amplification of gene were conducted by HRM technique. DnaSP v.5.0 was used for compression of diversity indices of ribosomal and mitochondrial markers of spp. The taxonomic status of spp. was performed by sequencing and hylogenetic analysis.
Overall, 26 and 4 isolates were identified as and , respectively. was the sole infecting species of revealed by HRM analysis. Genomic analysis showed a moderate ( genes: 0.600±0.215) to high (: 0.733±0.155) haplotype diversity for .
The parasite-dependent mitochondrial gene () could identify a higher genetic diversity of compared to nuclear gene. HRM technique in the present study found to be a reliable technique for identification and genetic diversity of liver flukes but more comprehensive and in-depth studies in different parts of the country are needed.
该物种和其他物种的肝吸虫被认为是家养和野生食草动物的寄生虫。这两个物种以及另一种都分布在伊朗。本研究旨在通过高分辨率熔解曲线（HRM）方法，利用线粒体细胞色素c氧化酶I（cytochrome c oxidase I）基因鉴定某些物种。
2022年，从伊朗西北部加兹温省的屠宰场收集了30份受感染的肝脏标本，其中来自绵羊的有23份，来自牛的有7份。通过HRM技术进行DNA提取和细胞色素c氧化酶I基因的PCR扩增。使用DnaSP v.5.0压缩某些物种核糖体和线粒体标记的多样性指数。通过测序和系统发育分析确定某些物种的分类地位。
总体而言，分别鉴定出26株和4株分离株为某些物种和另一些物种。HRM分析显示，某物种是唯一感染的物种。基因组分析表明，该物种的单倍型多样性从中等（细胞色素c氧化酶I基因：0.600±0.215）到高（另一种：0.733±0.155）。
与核基因相比，依赖寄生虫的线粒体基因（细胞色素c氧化酶I）可以鉴定出更高的某物种遗传多样性。本研究中的HRM技术被发现是一种用于鉴定肝吸虫及其遗传多样性的可靠技术，但需要在该国不同地区进行更全面和深入的研究。
