• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-149-5p 通过靶向组蛋白去乙酰化酶 4（HDAC4）抑制血管平滑肌细胞增殖、侵袭和迁移。

miR-149-5p Inhibits Vascular Smooth Muscle Cells Proliferation, Invasion, and Migration by Targeting Histone Deacetylase 4 (HDAC4).

机构信息

Department of Cardiology, Tianjin Nankai Hospital, Tianjin, China (mainland).

Department of Toxicology, Technical Center for Safety of Industrial Products, Tianjin Entry Exit Inspection and Quarantine Bureau, Dongli, Tianjin, China (mainland).

出版信息

Med Sci Monit. 2019 Oct 9;25:7581-7590. doi: 10.12659/MSM.916522.

DOI:10.12659/MSM.916522
PMID:31595884
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6796703/
Abstract

BACKGROUND Studies have demonstrated that microRNAs (miRNAs) have essential roles in biological functions of vascular smooth muscle cells (VSMCs). However, the function and related molecular mechanism of miR-149-5p in VSMCs remains unclear. MATERIAL AND METHODS We used MTT assay, Transwell assay, and wound-healing assay to measure the proliferation, invasion, and migration of VSMCs transfected with miR-149-5p mimics or inhibitors, respectively. Bioinformatics tools and luciferase assay were used to validate the relationship between miR-149-5p and histone deacetylase 4 (HDAC4). Rescue experiments were used to confirm the interaction of miR-149-5p and HDAC4 in regulating biological functions in VSMCs. RESULTS miR-149-5p was downregulated in PDGF-bb-induced VSMCs. It was also found that miR-149-5p overexpression suppressed proliferation, invasion, and migration of VSMCs, while miR-149-5p knockdown showed the opposite effects. Furthermore, HDAC4 was found to be a potential target of miR-149-5p, which rescued miR-149-5p-mediated proliferation, invasion, and migration in VSMCs. CONCLUSIONS We demonstrated that miR-149-5p can suppress biological functions of VSMCs by regulating HDAC4, which might provide a potent therapeutic target for VSMC growth-related diseases.

摘要

背景

研究表明，微小 RNA（miRNAs）在血管平滑肌细胞（VSMCs）的生物学功能中发挥着重要作用。然而，miR-149-5p 在 VSMCs 中的功能和相关分子机制尚不清楚。

材料与方法

我们分别使用 MTT 检测、Transwell 检测和划痕愈合检测来测量转染 miR-149-5p 模拟物或抑制剂的 VSMCs 的增殖、侵袭和迁移。生物信息学工具和荧光素酶报告基因检测用于验证 miR-149-5p 与组蛋白去乙酰化酶 4（HDAC4）之间的关系。采用挽救实验来确认 miR-149-5p 和 HDAC4 在调节 VSMCs 生物学功能中的相互作用。

结果

PDGF-bb 诱导的 VSMCs 中 miR-149-5p 表达下调。研究还发现，miR-149-5p 过表达抑制了 VSMCs 的增殖、侵袭和迁移，而 miR-149-5p 敲低则表现出相反的效果。此外，发现 HDAC4 是 miR-149-5p 的潜在靶标，可挽救 miR-149-5p 介导的 VSMCs 增殖、侵袭和迁移。

结论

我们证实，miR-149-5p 通过调节 HDAC4 抑制 VSMCs 的生物学功能，这可能为 VSMC 生长相关疾病提供一个有效的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/4bab6ef1d9a7/medscimonit-25-7581-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/40419ac644ff/medscimonit-25-7581-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/1ef651b40834/medscimonit-25-7581-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/ad17b254029c/medscimonit-25-7581-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/4ec7c622a691/medscimonit-25-7581-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/44410690e37b/medscimonit-25-7581-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/4bab6ef1d9a7/medscimonit-25-7581-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/40419ac644ff/medscimonit-25-7581-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/1ef651b40834/medscimonit-25-7581-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/ad17b254029c/medscimonit-25-7581-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/4ec7c622a691/medscimonit-25-7581-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/44410690e37b/medscimonit-25-7581-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f285/6796703/4bab6ef1d9a7/medscimonit-25-7581-g006.jpg

相似文献

1
miR-149-5p Inhibits Vascular Smooth Muscle Cells Proliferation, Invasion, and Migration by Targeting Histone Deacetylase 4 (HDAC4).miR-149-5p 通过靶向组蛋白去乙酰化酶 4（HDAC4）抑制血管平滑肌细胞增殖、侵袭和迁移。
Med Sci Monit. 2019 Oct 9;25:7581-7590. doi: 10.12659/MSM.916522.
2
Interfering histone deacetylase 4 inhibits the proliferation of vascular smooth muscle cells via regulating MEG3/miR-125a-5p/IRF1.组蛋白去乙酰化酶 4 的干扰通过调节 MEG3/miR-125a-5p/IRF1 抑制血管平滑肌细胞的增殖。
Cell Adh Migr. 2019 Dec;13(1):41-49. doi: 10.1080/19336918.2018.1506653. Epub 2018 Aug 29.
3
Circ_CHFR Promotes Platelet-Derived Growth Factor-BB-Induced Proliferation, Invasion, and Migration in Vascular Smooth Muscle Cells via the miR-149-5p/NRP2 Axis.环状 CHFR 通过 miR-149-5p/NRP2 轴促进血小板衍生生长因子-BB 诱导的血管平滑肌细胞增殖、侵袭和迁移。
J Cardiovasc Pharmacol. 2022 Jan 1;79(1):e94-e102. doi: 10.1097/FJC.0000000000001055.
4
Circ_0002984 Enhances Growth, Invasion, and Migration in PDGF-bb-Induced Vascular Smooth Muscle Cells Through miR-379-5p/FRS2 Axis.Circ_0002984 通过 miR-379-5p/FRS2 轴增强 PDGF-bb 诱导的血管平滑肌细胞的生长、侵袭和迁移。
J Cardiovasc Pharmacol. 2021 Dec 1;78(6):875-884. doi: 10.1097/FJC.0000000000001143.
5
MiR-155-5p inhibits the proliferation and migration of VSMCs and HUVECs in atherosclerosis by targeting AKT1.miR-155-5p 通过靶向 AKT1 抑制动脉粥样硬化中 VSMCs 和 HUVECs 的增殖和迁移。
Eur Rev Med Pharmacol Sci. 2019 Mar;23(5):2223-2233. doi: 10.26355/eurrev_201903_17270.
6
lncRNA GAS5 acts as a ceRNA for miR-21 in suppressing PDGF-bb-induced proliferation and migration in vascular smooth muscle cells.长链非编码 RNA GAS5 通过竞争性结合 miR-21 抑制 PDGF-bb 诱导的血管平滑肌细胞增殖和迁移。
J Cell Biochem. 2019 Sep;120(9):15233-15240. doi: 10.1002/jcb.28789. Epub 2019 May 8.
7
miR-145-5p Inhibits Vascular Smooth Muscle Cells (VSMCs) Proliferation and Migration by Dysregulating the Transforming Growth Factor-b Signaling Cascade.miR-145-5p 通过扰乱转化生长因子-β信号级联来抑制血管平滑肌细胞（VSMCs）的增殖和迁移。
Med Sci Monit. 2018 Jul 15;24:4894-4904. doi: 10.12659/MSM.910986.
8
DLEU2 modulates proliferation, migration and invasion of platelet-derived growth factor-BB (PDGF-BB)-induced vascular smooth muscle cells (VSMCs) via miR-212-5p/YWHAZ axis.DLEU2 通过 miR-212-5p/YWHAZ 轴调节血小板衍生生长因子-BB（PDGF-BB）诱导的血管平滑肌细胞（VSMCs）的增殖、迁移和侵袭。
Cell Cycle. 2022 Oct;21(19):2013-2026. doi: 10.1080/15384101.2022.2079175. Epub 2022 Jul 1.
9
MiR-147b influences vascular smooth muscle cell proliferation and migration via targeting YY1 and modulating Wnt/β-catenin activities.miR-147b 通过靶向 YY1 并调节 Wnt/β-catenin 活性来影响血管平滑肌细胞的增殖和迁移。
Acta Biochim Biophys Sin (Shanghai). 2018 Sep 1;50(9):905-913. doi: 10.1093/abbs/gmy086.
10
Circ_0004872 promotes platelet-derived growth factor-BB-induced proliferation, migration and dedifferentiation in HA-VSMCs via miR-513a-5p/TXNIP axis.Circ_0004872 通过 miR-513a-5p/TXNIP 轴促进血小板衍生生长因子-BB 诱导的 HA-VSMCs 增殖、迁移和去分化。
Vascul Pharmacol. 2021 Oct;140:106842. doi: 10.1016/j.vph.2021.106842. Epub 2021 Feb 13.

引用本文的文献

1
Dietary inulin supplementation in early gestation regulates uterine fluid exosomes and angiogenesis to improve embryo implantation in sows.妊娠早期补充膳食菊粉可调节子宫液外泌体和血管生成，以改善母猪的胚胎着床。
J Anim Sci Biotechnol. 2025 Aug 5;16(1):111. doi: 10.1186/s40104-025-01247-0.
2
Targeting PDGF/PDGFR Signaling Pathway by microRNA, lncRNA, and circRNA for Therapy of Vascular Diseases: A Narrow Review.靶向 PDGF/PDGFR 信号通路的 microRNA、lncRNA 和 circRNA 治疗血管疾病：一个狭窄的综述。
Biomolecules. 2024 Nov 14;14(11):1446. doi: 10.3390/biom14111446.
3
MicroRNA as potential biomarker for severity, progression, and therapeutic monitoring in animal models of limb-girdle muscular dystrophy: a systematic review.

本文引用的文献

1
Interfering histone deacetylase 4 inhibits the proliferation of vascular smooth muscle cells via regulating MEG3/miR-125a-5p/IRF1.组蛋白去乙酰化酶 4 的干扰通过调节 MEG3/miR-125a-5p/IRF1 抑制血管平滑肌细胞的增殖。
Cell Adh Migr. 2019 Dec;13(1):41-49. doi: 10.1080/19336918.2018.1506653. Epub 2018 Aug 29.
2
7-cyclopentyl-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-d] pyrimidin-4-ylamine inhibits the proliferation and migration of vascular smooth muscle cells by suppressing ERK and Akt pathways.7-环戊基-5-(4-苯氧基苯基)-7H-吡咯并[2,3-d]嘧啶-4-胺通过抑制ERK和Akt信号通路来抑制血管平滑肌细胞的增殖和迁移。
Eur J Pharmacol. 2017 Mar 5;798:35-42. doi: 10.1016/j.ejphar.2017.02.004. Epub 2017 Feb 6.
3
微小RNA作为肢带型肌营养不良动物模型中严重程度、疾病进展及治疗监测的潜在生物标志物：一项系统综述
Front Cell Neurosci. 2023 Dec 7;17:1233181. doi: 10.3389/fncel.2023.1233181. eCollection 2023.
4
Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression Model.阐明外周血单个核细胞中的组蛋白去乙酰化酶基因表达特征，这些特征与心脏基本功能相关，并通过逻辑回归模型有助于冠状动脉疾病的分类。
Biomedicines. 2023 Nov 1;11(11):2952. doi: 10.3390/biomedicines11112952.
5
Tumor-Suppressive and Oncogenic Roles of microRNA-149-5p in Human Cancers.miR-149-5p 在人类癌症中的抑瘤和致癌作用。
Int J Mol Sci. 2022 Sep 16;23(18):10823. doi: 10.3390/ijms231810823.
6
Knockdown of lncRNA H19 alleviates ox-LDL-induced HCAECs inflammation and injury by mediating miR-20a-5p/HDAC4 axis.长链非编码 RNA H19 通过调控 miR-20a-5p/HDAC4 轴减轻 ox-LDL 诱导的 HCAECs 炎症和损伤。
Inflamm Res. 2022 Sep;71(9):1109-1121. doi: 10.1007/s00011-022-01604-z. Epub 2022 Jul 19.
7
MicroRNAs (miRNAs) in Cardiovascular Complications of Rheumatoid Arthritis (RA): What Is New?类风湿关节炎心血管并发症的 microRNAs（miRNAs）：有何新进展？
Int J Mol Sci. 2022 May 8;23(9):5254. doi: 10.3390/ijms23095254.
8
Clinical role of serum histone deacetylase 4 measurement in acute ischemic stroke: Relation to disease risk, severity, and prognosis.血清组蛋白去乙酰化酶 4 测量在急性缺血性脑卒中的临床作用：与疾病风险、严重程度和预后的关系。
J Clin Lab Anal. 2022 May;36(5):e24372. doi: 10.1002/jcla.24372. Epub 2022 Mar 30.
9
A Review on the Role of miR-149-5p in the Carcinogenesis.miR-149-5p 在癌症发生中的作用研究进展
Int J Mol Sci. 2021 Dec 30;23(1):415. doi: 10.3390/ijms23010415.
10
miR-149 Suppresses the Proliferation and Metastasis of Human Gastric Cancer Cells by Targeting FOXC1.miR-149 通过靶向 FOXC1 抑制人胃癌细胞的增殖和转移。
Biomed Res Int. 2021 Dec 17;2021:1503403. doi: 10.1155/2021/1503403. eCollection 2021.
HDAC4 is expressed on multiple T cell lineages but dispensable for their development and function.
组蛋白去乙酰化酶4（HDAC4）在多个T细胞谱系中表达，但对其发育和功能并非必需。
Oncotarget. 2017 Mar 14;8(11):17562-17572. doi: 10.18632/oncotarget.15077.
4
Cathepsin H-Mediated Degradation of HDAC4 for Matrix Metalloproteinase Expression in Hepatic Stellate Cells: Implications of Epigenetic Suppression of Matrix Metalloproteinases in Fibrosis through Stabilization of Class IIa Histone Deacetylases.组织蛋白酶H介导肝星状细胞中HDAC4降解以促进基质金属蛋白酶表达：通过稳定IIa类组蛋白去乙酰化酶对基质金属蛋白酶进行表观遗传抑制在肝纤维化中的意义
Am J Pathol. 2017 Apr;187(4):781-797. doi: 10.1016/j.ajpath.2016.12.001. Epub 2017 Feb 1.
5
p38/Sp1/Sp4/HDAC4/BDNF Axis Is a Novel Molecular Pathway of the Neurotoxic Effect of the Methylmercury.p38/Sp1/Sp4/HDAC4/BDNF轴是甲基汞神经毒性作用的一种新型分子途径。
Front Neurosci. 2017 Jan 19;11:8. doi: 10.3389/fnins.2017.00008. eCollection 2017.
6
MicroRNA-32 promotes cell proliferation, migration and suppresses apoptosis in breast cancer cells by targeting FBXW7.微小RNA-32通过靶向F-盒蛋白7促进乳腺癌细胞的增殖、迁移并抑制其凋亡。
Cancer Cell Int. 2017 Jan 25;17:14. doi: 10.1186/s12935-017-0383-0. eCollection 2017.
7
Role of Phosphorylated HDAC4 in Stroke-Induced Angiogenesis.磷酸化组蛋白去乙酰化酶4在中风诱导血管生成中的作用
Biomed Res Int. 2017;2017:2957538. doi: 10.1155/2017/2957538. Epub 2017 Jan 3.
8
miR-433 reduces cell viability and promotes cell apoptosis by regulating MACC1 in colorectal cancer.微小RNA-433通过调节结直肠癌中的MACC1降低细胞活力并促进细胞凋亡。
Oncol Lett. 2017 Jan;13(1):81-88. doi: 10.3892/ol.2016.5445. Epub 2016 Nov 30.
9
Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels.小血管和大血管血管平滑肌细胞的分离与培养
Methods Mol Biol. 2017;1527:349-354. doi: 10.1007/978-1-4939-6625-7_27.
10
Functions of microRNA-143 in the apoptosis, invasion and migration of nasopharyngeal carcinoma.微小RNA-143在鼻咽癌凋亡、侵袭和迁移中的作用
Exp Ther Med. 2016 Dec;12(6):3749-3755. doi: 10.3892/etm.2016.3847. Epub 2016 Oct 27.