Effects of Insecticide Stress on Expression of Transporter Gene in the Brown Planthopper, .

The brown planthopper (BPH), , is an important pest of rice that severely affects production. Insecticides are an important means of controlling BPH, but their long-term use has led to resistance. To provide insight into BPH responses to insecticide stress, we determined the expression levels of BPH transporter genes under treatment with thiamethoxam, abamectin, and cyantraniliprole at LC, LC, LC, and LC. We cloned 13 BPH transporters, named to . Conservative domain analysis showed that all 13 transporters have one nucleotide binding domain and one transmembrane domain, typical of semi-molecular transporters. Real-time quantitative PCR showed that thiamethoxam, abamectin, and cyantraniliprole stress increased the expression of some transporters gene in BPH. However, after treatment with thiamethoxam at LC and abamectin at LC, there was no significant upregulation of . These results indicate that the expression of varies in response to stress from different insecticides. These findings provide baseline information for further understanding of the molecular mechanisms of insecticide resistance in BPH.