Yang Hong, Zhou Cao, Yang Xi-Bin, Long Gui-Yun, Jin Dao-Chao
Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Institute of Entomology, Guizhou University, Guiyang 550025, China.
College of Tobacco Science, Guizhou University, Guiyang 550025, China.
Insects. 2019 Oct 8;10(10):334. doi: 10.3390/insects10100334.
The brown planthopper (BPH), , is an important pest of rice that severely affects production. Insecticides are an important means of controlling BPH, but their long-term use has led to resistance. To provide insight into BPH responses to insecticide stress, we determined the expression levels of BPH transporter genes under treatment with thiamethoxam, abamectin, and cyantraniliprole at LC, LC, LC, and LC. We cloned 13 BPH transporters, named to . Conservative domain analysis showed that all 13 transporters have one nucleotide binding domain and one transmembrane domain, typical of semi-molecular transporters. Real-time quantitative PCR showed that thiamethoxam, abamectin, and cyantraniliprole stress increased the expression of some transporters gene in BPH. However, after treatment with thiamethoxam at LC and abamectin at LC, there was no significant upregulation of . These results indicate that the expression of varies in response to stress from different insecticides. These findings provide baseline information for further understanding of the molecular mechanisms of insecticide resistance in BPH.
褐飞虱是水稻的一种重要害虫，严重影响水稻产量。杀虫剂是防治褐飞虱的重要手段，但其长期使用已导致褐飞虱产生抗性。为深入了解褐飞虱对杀虫剂胁迫的反应，我们测定了噻虫嗪、阿维菌素和氯虫苯甲酰胺在LC、LC、LC和LC处理下褐飞虱转运蛋白基因的表达水平。我们克隆了13个褐飞虱转运蛋白，命名为至。保守结构域分析表明，所有13个转运蛋白都有一个核苷酸结合结构域和一个跨膜结构域，这是半分子转运蛋白的典型特征。实时定量PCR表明，噻虫嗪、阿维菌素和氯虫苯甲酰胺胁迫增加了褐飞虱中一些转运蛋白基因的表达。然而，在LC浓度的噻虫嗪和LC浓度的阿维菌素处理后，并没有显著上调。这些结果表明，不同杀虫剂胁迫下转运蛋白的表达存在差异。这些发现为进一步了解褐飞虱抗药性的分子机制提供了基础信息。
