Zhou Cao, Yang Hong, Wang Zhao, Long Gui-Yun, Jin Dao-Chao
Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Institute of Entomology, Guizhou University, Guiyang, China.
College of Tobacco Science of Guizhou University, Guiyang, China.
Front Physiol. 2019 Jan 8;9:1890. doi: 10.3389/fphys.2018.01890. eCollection 2018.
, an important migratory pest of rice, has substantial detrimental effects on rice production. To clarify the mechanism whereby responds to insecticide stress, we measured the activity of its protective [superoxide dismutase (SOD); peroxidase (POD); catalase (CAT)] and detoxifying [carboxylesterase (CarE); glutathione -transferase (GST); mixed-function oxidase (MFO)] enzymes and the expression levels of its ATP-binding cassette subfamily G () transporter genes in response to sublethal concentrations (LC and LC) of the insecticides thiamethoxam, buprofezin, and abamectin. On the bases of the transcriptome data and the genes of , we obtained 14 full-length sequences for . RT-qPCR results showed that 13, 12, and 9 genes were upregulated in the presence of thiamethoxam, buprofezin, and abamectin, respectively, at LC. Moreover, 13 and 7 genes were upregulated following treatment with thiamethoxam and abamectin, respectively, at LC. Enzyme activity assays showed that although thiamethoxam, buprofezin, and abamectin induced GST, CarE, CAT, POD, and SOD activity, they did so at different concentrations and exposure times. The activity of MFO was generally inhibited with prolonged exposure to the three insecticides, with the inhibitory effect being most significant at 72 h. These results indicate that differs in its response to different types or concentrations of insecticides. Taken together, our results lay the foundations for gaining a deeper understanding of the mechanisms underlying the adaptation of to different types of insecticides, which would be of considerable significance for the development of effective pest management strategies.
[害虫名称]是水稻的一种重要迁飞性害虫，对水稻生产有重大不利影响。为阐明[害虫名称]对杀虫剂胁迫的响应机制，我们测定了其保护性酶[超氧化物歧化酶（SOD）；过氧化物酶（POD）；过氧化氢酶（CAT）]和解毒酶[羧酸酯酶（CarE）；谷胱甘肽 -S-转移酶（GST）；多功能氧化酶（MFO）]的活性，以及其ATP结合盒亚家族G（[转运蛋白名称]）转运蛋白基因在亚致死浓度（LC[X]和LC[Y]）的噻虫嗪、噻嗪酮和阿维菌素作用下的表达水平。基于转录组数据和[害虫名称]的[转运蛋白名称]基因，我们获得了14条[转运蛋白名称]的全长序列。RT-qPCR结果表明，在LC[X]浓度下，分别有13、12和9个[转运蛋白名称]基因在噻虫嗪、噻嗪酮和阿维菌素存在时上调。此外，在LC[Y]浓度下，分别有13和7个[转运蛋白名称]基因在噻虫嗪和阿维菌素处理后上调。酶活性测定表明，尽管噻虫嗪、噻嗪酮和阿维菌素诱导了GST、CarE、CAT、POD和SOD的活性，但它们在不同浓度和暴露时间下诱导程度不同。随着暴露于这三种杀虫剂时间的延长，MFO的活性总体上受到抑制，在72小时时抑制作用最为显著。这些结果表明，[害虫名称]对不同类型或浓度的杀虫剂的反应存在差异。综上所述，我们的结果为深入了解[害虫名称]对不同类型杀虫剂的适应机制奠定了基础，这对于制定有效的害虫管理策略具有重要意义。
