Department of Medical Sciences, Uppsala University, Uppsala University Hospital, Uppsala, Sweden.
Institution of Statistics, Uppsala University, Uppsala, Sweden.
J Interferon Cytokine Res. 2020 Feb;40(2):71-74. doi: 10.1089/jir.2019.0074. Epub 2019 Oct 10.
There exists a close relationship between cardiovascular diseases and chronic kidney disease. Apolipoprotein A1 and high-density lipoprotein (HDL) cholesterol are widely used as cardiovascular risk markers but they also have anti-inflammatory properties. The aim of this study was to investigate any associations between HDL levels and cytokine levels in urine. We randomly selected 90 urine samples from the Prospective Investigation of the Vasculature in Uppsala Seniors Study (41 males and 49 females). The samples were analyzed with 2 multiplex assays, Multiplex Inflammation I and Cardiovascular II kits (Olink Bioscience, Uppsala, Sweden). We analyzed the correlations between 158 cytokines in urine with apolipoprotein A1, HDL cholesterol, apolipoprotein B, and low-density lipoprotein cholesterol. There were strong correlations for apolipoprotein A1 and HDL cholesterol with individual cytokines. After adjustment for multiplicity testing, there were 33 significant correlations between apolipoprotein A1 and cytokine levels and 14 of these were also significantly correlated with HDL cholesterol. The strongest associations were observed for IL-1α, SPON2, RAGE, PAR-1, TRAIL-R2, IL-4RA, TNFRSF11A, and SCF. A total of 28 out of 33 correlations were negative, indicating a negative relationship between apolipoprotein A1 and urinary cytokines. The study shows a negative correlation between apolipoprotein A1 and HDL cholesterol and urinary cytokine levels. The finding is in agreement with the anti-inflammatory properties of HDL.
心血管疾病与慢性肾脏病之间存在密切关系。载脂蛋白 A1 和高密度脂蛋白（HDL）胆固醇广泛用作心血管风险标志物，但它们也具有抗炎特性。本研究旨在探讨 HDL 水平与尿液中细胞因子水平之间的任何关联。我们从乌普萨拉老年人前瞻性血管研究（Prospective Investigation of the Vasculature in Uppsala Seniors Study）中随机选择了 90 个尿液样本（41 名男性和 49 名女性）。使用 Multiplex Inflammation I 和 Cardiovascular II 试剂盒（Olink Bioscience，瑞典乌普萨拉）对这些样本进行了分析。我们分析了尿液中 158 种细胞因子与载脂蛋白 A1、HDL 胆固醇、载脂蛋白 B 和低密度脂蛋白胆固醇之间的相关性。载脂蛋白 A1 和 HDL 胆固醇与个别细胞因子之间存在很强的相关性。在进行多重检验调整后，载脂蛋白 A1 与细胞因子水平之间存在 33 个显著相关性，其中 14 个与 HDL 胆固醇也呈显著相关性。最强的关联发生在 IL-1α、SPON2、RAGE、PAR-1、TRAIL-R2、IL-4RA、TNFRSF11A 和 SCF 上。在 33 个相关性中，有 28 个呈负相关，表明载脂蛋白 A1 与尿液细胞因子之间呈负相关。该研究表明载脂蛋白 A1 与 HDL 胆固醇和尿液细胞因子水平之间存在负相关。这一发现与 HDL 的抗炎特性一致。
