Jian Jiao, Liang Xu
Institutes of Science and Development, Chinese Academy of Sciences, Beijing 100190, China.
Beijing DaXing District Forestry Workstation, No. 17 Administrative Street, Huangcun Town, Beijing 102600, China.
Microorganisms. 2019 Oct 9;7(10):425. doi: 10.3390/microorganisms7100425.
The pathogenic fungus (), causing head blight (FHB) or scab, is one of the most important cereal killers worldwide, exerting great economic and agronomic losses on global grain production. To repress pathogen invasion, plants have evolved a sophisticated innate immunity system for pathogen recognition and defense activation. Simultaneously, pathogens continue to evolve more effective means of invasion to conquer plant resistance systems. In the process of co-evolution of plants and pathogens, several small RNAs (sRNAs) have been proved in regulating plant immune response and plant-microbial interaction. In this study, we report that a sRNA () can suppress wheat defense response by targeting and silencing a resistance-related gene, which codes a Chitin Elicitor Binding Protein (). Transcriptional level evidence indicates that can target mRNA and trigger silencing of in vivo, and in () plants, Western blotting experiments and YFP Fluorescence observation proofs show that can suppress the accumulation of protein coding by gene in vitro. PH-1 strain displays a weakening ability to invasion when Barley stripe mosaic virus (BSMV) vector induces effective silencing in PH-1 infected wheat plants. Taken together, our results suggest that a small RNA from can target and silence the wheat gene to enhance invasion of .
引起赤霉病的致病真菌（）是全球最重要的谷物杀手之一，给全球粮食生产造成了巨大的经济和农艺损失。为了抑制病原体入侵，植物进化出了一套复杂的先天免疫系统来识别病原体并激活防御。同时，病原体也在不断进化出更有效的入侵手段来攻克植物的抗性系统。在植物与病原体的共同进化过程中，已证实几种小RNA（sRNAs）在调节植物免疫反应和植物-微生物相互作用中发挥作用。在本研究中，我们报道了一种sRNA（）可通过靶向并沉默一个编码几丁质激发子结合蛋白（）的抗性相关基因来抑制小麦的防御反应。转录水平的证据表明，可在体内靶向mRNA并触发的沉默，并且在（）植物中，蛋白质印迹实验和YFP荧光观察证明可在体外抑制基因编码的蛋白质的积累。当大麦条纹花叶病毒（BSMV）载体在感染PH-1的小麦植株中诱导有效的沉默时，PH-1菌株的入侵能力减弱。综上所述，我们的结果表明，来自的一种小RNA可靶向并沉默小麦基因以增强的入侵。
