McCurrach K, Glover L A, Hardman N
Department of Biochemistry, University of Aberdeen, Marischal College, Scotland.
Curr Genet. 1988;13(1):71-4. doi: 10.1007/BF00365759.
A plasmid was constructed containing a replication origin sequence from the Physarum ribosomal DNA molecule, and a bacterial chloramphenicol acetyltransferase (CAT) gene linked to a putative promoter of the long terminal repeat (LTR) of the Physarum "HpaII-repeat" element. The plasmid was transfected into Physarum myxamoebae either by electroporation or CaCl2 treatment. In both cases significant transient levels of CAT gene expression were detected. Results were compared with those obtained with plasmids in which CAT gene expression was driven by eukaryotic virus promoters.
构建了一种质粒，其包含来自黏菌核糖体DNA分子的复制起始序列，以及与黏菌“ HpaII重复”元件的长末端重复序列（LTR）的假定启动子相连的细菌氯霉素乙酰转移酶（CAT）基因。通过电穿孔或氯化钙处理将该质粒转染到黏菌变形虫中。在这两种情况下，均检测到CAT基因表达的显著瞬时水平。将结果与用真核病毒启动子驱动CAT基因表达的质粒所获得的结果进行比较。
